Guinea pig Anti-NaV1.8 (SCN10A) Antibody

PN3, SNS, Sodium channel protein type 10 subunit alpha
    Cat #: AGP-029
    Alternative Name PN3, SNS, Sodium channel protein type 10 subunit alpha
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Guinea pig
    Reactivity: r
    May also work in: h*, m*
    Immunogen
    • Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Accession Q63554). Intracellular, C-terminus.
    • Guinea pig Anti-NaV1.8 (SCN10A) Antibody
    Accession (Uniprot) Number Q63554
    Gene ID 29571
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.
    RRID AB_2340965.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Guinea pig total IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Guinea pig Anti-NaV1.8 (SCN10A) Antibody
      Western blot analysis of rat DRG lysate:
      1. Guinea pig Anti-NaV1.8 (SCN10A) Antibody (#AGP-029), (1:200).
      2. Guinea pig Anti-NaV1.8 (SCN10A) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Guinea pig Anti-NaV1.8 (SCN10A) Antibody
      Expression of NaV1.8 in rat DRG
      Immunohistochemical staining of rat dorsal root ganglion (DRG) using Guinea pig Anti-NaV1.8 (SCN10A) Antibody (#AGP-029). A. NaV1.8 staining (green) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.
    References
    1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    2. Baker, M.D. et al. (2001) Trends Pharmacol. Sci. 22, 27.
    3. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
    4. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
    5. Fjell, J. et al. (2000) NeuroReport 11, 199.
    6. Renganathan, M. et al. (2003) Brain Res. 959, 235.
    Scientific background

    Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.92,3. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels4,5. The expression of the α subunit isoform is developmentally regulated and tissue specific.

    Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons4-6. TTX-resistant channels have been suggested to play an important role in nociceptive transmission.

    Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat NaV1.8 channel. Guinea pig Anti-NaV1.8 (SCN10A) Antibody (#AGP-029), raised in guinea pigs can be used in western blot and immunohistochemistry applications. It has been designed to recognize NaV1.8 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-NaV1.8 (SCN10A) Antibody (#ASC-016) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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