Name: Guinea pig Anti-NaV1.8 (SCN10A) Antibody
Brand: Alomone Labs
SKU: ASC-016-GP (formerly AGP-029)

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Overview

Cat #: ASC-016-GP (formerly AGP-029)

Alternative Name PN3, SNS, Sodium channel protein type 10 subunit alpha

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: r

May also work in: h*, m*

Immunogen

Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat Na_V1.8 (Accession Q63554). Intracellular, C-terminus.

Accession (Uniprot) Number Q63554

Gene ID 29571

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.

RRID AB_2340965.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Rat DRG lysate (1:200-1:1000).
Western blot analysis of rat DRG lysate:
1. Guinea pig Anti-Na_V1.8 (SCN10A) Antibody (#ASC-016-GP), (1:200).
2. Guinea pig Anti-Na_V1.8 (SCN10A) Antibody, preincubated with Nav1.8/SCN10A Blocking Peptide (#BLP-SC016).
Following a broad screen of secondary antibodies, the following was used for this application: #106-035-006 (Jackson ImmunoResearch).

Immunohistochemistry

Rat DRG. Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).

Scientific background

Voltage-gated Na⁺ channels (Na_V) are essential for the generation of action potentials and for cell excitability¹. Na_V channels are activated in response to depolarization and selectively allow flow of Na⁺ ions. To date, nine Na_V α subunits have been cloned and named Na_V1.1-1.9^2,3. The Na_V channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels^4,5. The expression of the α subunit isoform is developmentally regulated and tissue specific.

Two TTX-resistant Na_V channels are expressed in dorsal root ganglion (DRG) neurons, Na_V1.8 and Na_V1.9. The Na_V1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons^4-6. TTX-resistant channels have been suggested to play an important role in nociceptive transmission.

Recently, involvement of Na_V1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 06/09/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Nav1.8/SCN10A Blocking Peptide (#BLP-SC016) is the original antigen used for immunization during Anti-Na_V1.8 (SCN10A) Antibody (#ASC-016) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.8/SCN10A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Nav1.8/SCN10A Blocking Peptide (#BLP-SC016)

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Certificate of Analysis

Guinea pig Anti-Na_V1.8 (SCN10A) Antibody