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Guinea pig Anti-SCN11A (NaV1.9) Antibody

PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha
Cat #: AGP-030
Alternative Name PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: r
May also work in: h*, m*
Immunogen
  • Peptide CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9 (Accession O88457). Intracellular, C-terminus.
Accession (Uniprot) Number O88457
Gene ID 29701
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 15/18 amino acid residues identical; human - 14/18 amino acid residues identical.
RRID AB_2340966.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain membrane:
    Western blot analysis of rat brain membrane:
    1. Guinea pig Anti-SCN11A (NaV1.9) Antibody (#AGP-030), (1:200).
    2. Guinea pig Anti-SCN11A (NaV1.9) Antibody, preincubated with SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017).
Immunohistochemistry
  • Expression of NaV1.9 in rat DRG
    Expression of NaV1.9 in rat DRG
    Immunohistochemical staining of NaV1.9 in rat dorsal root ganglion (DRG) using Guinea pig Anti-SCN11A (NaV1.9) Antibody (#AGP-030). A. NaV1.9 staining (green) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.
References
  1. Wu, L. et al. (2002) NeuroReport 13, 2547.
  2. Baker, M.D. et al. (2001) Trends Pharmacol. Sci. 22, 27.
  3. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
  4. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
  5. Fjell, J. et al. (2000) NeuroReport 11, 199.
  6. Blum, R. et al. (2002) Nature 419, 687.
Scientific background

Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.92,3. Their expression is developmentally and tissue specific.

NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels4,5. TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.9 channel (also called SCN11A, NAN or SNS2) is preferentially expressed in small-diameter DRG neurons4,5.

Recently, it has been shown that BDNF activated NaV1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat protein. Guinea pig Anti-SCN11A (NaV1.9) Antibody (#AGP-030), raised in guinea pigs, can be used in western blot and immunohistochemical applications, and has been designed to recognize NaV1.9 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-SCN11A (NaV1.9) Antibody (#ASC-017) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunohistochemistry citations
  1. Rat brain sections (5 µg/ml).
    Kurowski, P. et al. (2015) Neuroscience 303, 474.
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