- Peptide CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9 (Accession O88457). Intracellular, C-terminus.
- Western blot analysis of rat brain membrane:1. Guinea pig Anti-SCN11A (NaV1.9) Antibody (#AGP-030), (1:200).
2. Guinea pig Anti-SCN11A (NaV1.9) Antibody, preincubated with SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017).
- Expression of NaV1.9 in rat DRGImmunohistochemical staining of NaV1.9 in rat dorsal root ganglion (DRG) using Guinea pig Anti-SCN11A (NaV1.9) Antibody (#AGP-030). A. NaV1.9 staining (green) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.
Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.92,3. Their expression is developmentally and tissue specific.
NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels4,5. TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.9 channel (also called SCN11A, NAN or SNS2) is preferentially expressed in small-diameter DRG neurons4,5.
Recently, it has been shown that BDNF activated NaV1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone6.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat protein. Guinea pig Anti-SCN11A (NaV1.9) Antibody (#AGP-030), raised in guinea pigs, can be used in western blot and immunohistochemical applications, and has been designed to recognize NaV1.9 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-SCN11A (NaV1.9) Antibody (#ASC-017) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.