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Anti-Nectin-1/PVRL1 (extracellular)-FITC Antibody

Poliovirus receptor-related protein 1, Herpes virus entry mediator C, PRR1, CD111

Cat #: ANR-051-F
Alternative Name Poliovirus receptor-related protein 1, Herpes virus entry mediator C, PRR1, CD111
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GKPPSVVSWETRLK, corresponding to amino acid residues 177-190 of human Nectin-1 (Accession Q15223). Extracellular, N-terminus.
Accession (Uniprot) Number Q15223
Gene ID 5818
Homology Rat - identical; mouse – 13 out of 14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANR-051), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of Nectin-1 by direct flow cytometry in live intact mouse J774 macrophage cells:
    Cell surface detection of Nectin-1 by direct flow cytometry in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Nectin-1/PVRL1 (extracellular)-FITC Antibody (#ANR-051-F), (2.5µg).
  • Cell surface detection of Nectin-1 by direct flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells:
    Cell surface detection of Nectin-1 by direct flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Nectin-1/PVRL1 (extracellular)-FITC Antibody (#ANR-051-F), (5µg).
  • Cell surface detection of Nectin-1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of Nectin-1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Nectin-1/PVRL1 (extracellular)-FITC Antibody (#ANR-051-F), (2.5µg).
References
  1. Rikitake, Y. et al. (2012) J. Cell Sci. 125, 3713.
  2. Fantin, M. et al. (2013) PLoS One 8, e56897.
  3. Wakamatsu, K. et al. (2007) J. Biol. Chem282, 1817.
  4. Sözen, M.A. et al. (2001) Nat. Genet. 29, 141.
  5. Suzuki, K. et al. (2000) Nat. Genet. 25, 427.
Scientific background

Nectins, which were originally identified as virus receptors, are members of the cell-cell adhesion molecule (CAM) family. They are Ca2+-independent immunoglobulin-like CAMs. The nectin family comprises four members, nectin-1, nectin-2, nectin-3 and nectin-4, which are encoded by the PVRL1, PVRL2, PVRL3 and PVRL4 genes, respectively1.  In the central nervous system, these cell adhesion molecules aggregate in formations, termed puncta adherentia junctions, which are mechanical adhesive sites that connect pre- and postsynaptic membranes2.

All nectins contain an extracellular region with three immunoglobulin-like loops (one V type and two C2 types), a single membrane-spanning region and a cytoplasmic tail. Nectins directly bind afadin, an F-actin-binding protein, through their cytoplasmic tails1.

Nectin-1 is expressed at cell-cell junctions in human and mouse epidermis. In mice lacking the gene encoding nectin-1 (Pvrl1−/− mice), the expression of loricrin, a differentiation marker and a major component of cornified cell envelopes in the epidermis, is downregulated and newborn pups have a shiny and slightly reddish skin3. Mutations in human PVRL1 are implicated in cleft lip or palate-ectodermal dysplasia syndromes, which includes Zlotogora–Ogur syndrome and Margarita Island ectodermal dysplasia4. Both of these are autosomal recessive disorders that are clinically characterized by unusual facial appearance, dental anomalies, hypotrichosis, palmoplantar hyperkeratosis and onychodysplasia, syndactyly, cleft lip or palate, and in some cases, mental retardation5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/05/2021

Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize Nectin-1 from human, mouse and rat samples. It will recognize all known isoforms of Nectin-1, including the secreted form (also known as isoform gamma).

Anti-Nectin-1/PVRL1 (extracellular)-FITC Antibody (#ANR-051-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

For research purposes only, not for human use
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