Anti-Netrin-1 Antibody

NTN1, MRMV4
  • New
Cat #: ANR-121
Alternative Name NTN1, MRMV4
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CRKMYNRPHRAPITK, corresponding to amino acid residues 181 - 195 of rat Netrin-1 (Accession Q924Z9). Secreted.
    • Anti-Netrin-1 Antibody
    Accession (Uniprot) Number Q924Z9
    Gene ID 114523
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ih*, ip*
    Western blot
    • Anti-Netrin-1 Antibody
      Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):
      1, 2. Anti-Netrin-1 Antibody (#ANR-121), (1:200).
      3, 4. Anti-Netrin-1 Antibody, preincubated with the negative control antigen.
    • Anti-Netrin-1 Antibody
      Western blot analysis of human U-87 MG glioblastoma cell line lysates (lanes 1 and 3) and human PANC-1 pancreas ductal adenocarcinoma cell line lysates (lanes 2 and 4):
      1, 2. Anti-Netrin-1 Antibody (#ANR-121), (1:200).
      3, 4. Anti-Netrin-1 Antibody, preincubated with the negative control antigen.
    References
    1. Serafini, T. et al. (1996) Cell 87, 1001.
    2. Kennedy, T.E. et al. (2006) J. Neurosci. 26, 8866.
    3. Kennedy, T.E. et al. (1994) Cell 78, 425.
    4. Boyer, N.P and Gupton, S.L. (2018) Front. Cell. Neurosci. 12, 221.
    Scientific background

    Netrins are a family comprised of five members: three secreted proteins (netrins 1, 3 and 4), and two glycosylphosphatidylinositol (GPI)-anchored membrane proteins, netrins G1 and G21. Netrin-1 is a diffusible long-range axonal guidance cue expressed in floor plate cells of the central nervous system. It is secreted in order to direct exons to their target1-3.  Axon guidance by netrin-1 is implicated in multiple developing brain regions and developing neuronal types. It also plays a role in axon branching, synaptogenesis, cell migration, cell survival, and axon regeneration4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 07/06/2020

    Anti-Netrin-1 Antibody (#ANR-121) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Netrin-1 from human, mouse, and rat samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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