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Anti-Netrin-1 Antibody

NTN1, MRMV4

Cat #: ANR-121
Alternative Name NTN1, MRMV4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CRKMYNRPHRAPITK, corresponding to amino acid residues 181 - 195 of rat Netrin-1 (Accession Q924Z9). Secreted.
Accession (Uniprot) Number Q924Z9
Gene ID 114523
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2876817
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):
    Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):
    1, 2. Anti-Netrin-1 Antibody (#ANR-121), (1:200).
    3, 4. Anti-Netrin-1 Antibody, preincubated with Netrin-1 Blocking Peptide (#BLP-NR121).
  • Western blot analysis of human U-87 MG glioblastoma cell line lysates (lanes 1 and 3) and human PANC-1 pancreas ductal adenocarcinoma cell line lysates (lanes 2 and 4):
    Western blot analysis of human U-87 MG glioblastoma cell line lysates (lanes 1 and 3) and human PANC-1 pancreas ductal adenocarcinoma cell line lysates (lanes 2 and 4):
    1, 2. Anti-Netrin-1 Antibody (#ANR-121), (1:200).
    3, 4. Anti-Netrin-1 Antibody, preincubated with Netrin-1 Blocking Peptide (#BLP-NR121).
Immunohistochemistry
  • Expression of Netrin-1 in mouse hippocampus.
    Expression of Netrin-1 in mouse hippocampus.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Netrin-1 Antibody (#ANR-121), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Netrin-1 immunoreactivity (green) appears in cells (horizontal arrows) in the hilus (H) in the hippocampal dentate gyrus. B. Pre-incubation of the antibody with Netrin-1 Blocking Peptide (BLP-NR121), suppressed staining. Cell nuclei are stained with DAPI (blue). G= granule layer, H = Hilus.
  • Expression of Netrin-1 in mouse cerebellum.
    Expression of Netrin-1 in mouse cerebellum.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Netrin-1 Antibody (#ANR-121), (1:300), followed by goat anti-rabbit-AlexaFluor-488. Netrin-1 immunoreactivity (green) appears in Purkinje cell soma (horizontal arrows) and in Purkinje dendrites (vertical arrows). B. Pre-incubation of the antibody with Netrin-1 Blocking Peptide (BLP-NR121), suppressed staining. Cell nuclei are stained with DAPI (blue). G= granule layer, P = Purkinje layer, M = molecular layer
References
  1. Serafini, T. et al. (1996) Cell 87, 1001.
  2. Kennedy, T.E. et al. (2006) J. Neurosci. 26, 8866.
  3. Kennedy, T.E. et al. (1994) Cell 78, 425.
  4. Boyer, N.P and Gupton, S.L. (2018) Front. Cell. Neurosci. 12, 221.
Scientific background

Netrins are a family comprised of five members: three secreted proteins (netrins 1, 3 and 4), and two glycosylphosphatidylinositol (GPI)-anchored membrane proteins, netrins G1 and G21. Netrin-1 is a diffusible long-range axonal guidance cue expressed in floor plate cells of the central nervous system. It is secreted in order to direct exons to their target1-3.  Axon guidance by netrin-1 is implicated in multiple developing brain regions and developing neuronal types. It also plays a role in axon branching, synaptogenesis, cell migration, cell survival, and axon regeneration4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2021

Anti-Netrin-1 Antibody (#ANR-121) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Netrin-1 from human, mouse, and rat samples.

For research purposes only, not for human use
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