Overview
- Peptide (C)KEDQWLEKKVQGNKDH, corresponding to amino acid residues 635-650 of mouse NCAM-2 (Accession O35136). Extracellular, N-terminus.
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody (#ANR-042), (1:200).
3,4. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody, preincubated with NCAM2 (extracellular) Blocking Peptide (#BLP-NR042).
Western blot analysis of human LNCaP prostate carcinoma (lanes 5 and 7) and MCF-7 breast adenocarcinoma (lanes 6 and 8) cell lysates:5,6. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody (#ANR-042), (1:200).
7,8. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody, preincubated with NCAM2 (extracellular) Blocking Peptide (#BLP-NR042).
Expression of NCAM2 in mouse brainImmunohistochemical staining of mouse free-floating frozen brain sections using Anti-NCAM2 (extracellular) Antibody (#ANR-042). A. In the hippocampal CA3 region, NCAM2 immunoreactivity (green) appears in the pyramidal layer (P, arrows). B. In the olfactory bulb, NCAM2 staining (green) appears in the Mitral cell layer (MCL, arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
Cell surface detection of NCAM2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:___ Cells.
___ Cells + goat-anti-rabbit-APC.
___ Cells + Anti-NCAM2 (extracellular) Antibody (#ANR-042), (2.5μg) + goat-anti-rabbit-APC.
- Leshchyns'ka, I. et al. (2015) Nat. Commun. 6, 8836.
- Kulahin, N. and Walmod, P.S. (2010) Adv. Exp. Med. Biol. 663, 403.
- Kulahin, N. et al. (2011) Structure 19, 203.
Neural cell adhesion molecule 2 (NCAM-2), a protein also known as olfactory cell adhesion molecule (OCAM), belongs to the immunoglobulin superfamily of cell adhesion molecules that participate in homophilic trans-interactions. NCAM-2 is highly expressed in the nervous system and participates in numerous cellular events, such as survival, migration, and differentiation, neurite outgrowth, and formation and plasticity of synapses1,2.
The NCAM-2 structure consists of five immunoglobulin (Ig) domains followed by two fibronectin-type 3 (Fn3) domains and eight potential sites for N-linked glycosylation3.
Overexpression of NCAM-2 is believed to be a contributing factor to Down syndrome. There is also a genetic association between NCAM-2 and Alzheimer's disease, where NCAM-2 is involved in the pathogenic pathway to the senile plaques that concentrate in the Alzheimer's disease brain1.
During human embryonic development, NCAM-2 is expressed in several tissues, including lung, liver, and kidney with the highest expression in the brain. NCAM-2 expression level rises around postnatal day 21 and remains high during adulthood. Studies conducted on NCAM-2 deficient mice show that the protein is important for the development of the brain and the olfactory system1.
Anti-NCAM2 (extracellular) Antibody (#ANR-042) is a highly specific antibody directed against mouse Neural cell adhesion molecule 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NCAM2 from rat, mouse, and human samples.
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