Anti-Neuroligin 2 (extracellular) Antibody

NLGN2, NL2
    Cat #: ANR-036
    Alternative Name NLGN2, NL2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    Immunogen
    • Peptide (C)DLGPRAYDRFPGDS, corresponding to amino acid residues 657-670 of rat NLGN2 (Accession Q62888). Extracellular, N-terminus.
    • Anti-Neuroligin 2 (extracellular) Antibody
    Accession (Uniprot) Number Q62888
    Gene ID 117096
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 12/14 amino acid residues identical.
    RRID AB_2341007.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-Neuroligin 2 (extracellular) Antibody
      Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and rat PC12 cells (lanes 3 and 6):
      1-3. Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036), (1:400).
      4-6. Anti-Neuroligin 2 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-Neuroligin 2 (extracellular) Antibody
      Expression of NLGN2 in rat DRG
      Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. NLGN2 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.
    • Anti-Neuroligin 2 (extracellular) Antibody
      Expression of NLGN2 in rat brain
      Immunnohistochemical staining of rat reticular thalamic nucleus using Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036).  A. NLGN2 staining (red) is detected in neuronal outlines (arrow). B. Nucleus staining using DAPI as the counterstain. C. Merged images of A and B.
    Live cell imaging / Immunocytochemistry
    • Anti-Neuroligin 2 (extracellular) Antibody
      Expression of NLGN2 in rat PC12 cells
      Cell surface detection of NLGN2 in intact living rat PC12 cells. A. Extracellular staining of cells using Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036), (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of A and B.
    References
    1. Chubykin, A.A. et al. (2007) Neuron 54, 919.
    2. Kohl, C. et al. (2013) PLoS ONE 8, e5687.
    3. Suckow, A.T. et al. (2008) Endocrinology 149, 6006.
    4. Lee, K. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 336.
    5. Sun C. et al. (2011) Hum. Mol. Genet. 20, 3042.
    Scientific background

    Neuroligins (NLGNs) are a family of postsynaptic cell adhesion molecules that play an important role in synaptic development and function1. Four genes encode for the different members of the neuroligin family in mammals: NLGN 1-4, which are differentially enriched in postsynaptic specializations of synapses2. Neuroligin 2 (NLGN2) is expressed in neurons in the brain and also in pancreatic β cells where it facilitates insulin secretion3.

    Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners3. NLGN2 plays a role in synapse function and synaptic signal transmission, especially via γ-aminobutyric acid receptors (GABA(A) receptors). NLGN2 modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing4.

    Recent studied identified a loss-of-function mutation of NLGN2 in schizophrenia patients, suggesting an indispensable role of NLGN2 in regulating GABAergic functions5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 25/02/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Neuroligin 2. Anti-Neuroligin 2 (extracellular) Antibody (#ANR-036) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NLGN2 from rat, mouse and human samples.

    For research purposes only, not for human use
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