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Anti-Neuronal Pentraxin 1 (NPTX1) Antibody

Neuronal Pentraxin-1, NP1

Cat #: ANR-191
Alternative Name Neuronal Pentraxin-1, NP1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)ERAKIESALTSLHQR, corresponding to amino acid residues 197 - 211 of mouse NPTX1 (Accession Q62443). Secreted.
Accession (Uniprot) Number Q62443
Gene ID 18164
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical; Human - 13 out of 15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1-2. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191), (1:400).
    3-4. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody, preincubated with Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (BLP-NR191).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):
    1-2. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191), (1:400).
    3-4. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody, preincubated with Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (BLP-NR191).
Immunohistochemistry
  • Expression of Neuronal Pentraxin 1 in rat striatum.
    Expression of Neuronal Pentraxin 1 in rat striatum.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NPTX1 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (BLP-NR191), suppressed staining. Cell nuclei are stained with DAPI (blue). CC = corpus callosum, ST = striatum.
References
  1. Ma, Q.L. et al (2018) Neurobiol Dis, 114, 120.
  2. Mir Ahamed Hossain (2008), Int J Dev Neurosci, 26, 93.
  3. Wang, Z. et al (2020) Front Immunol, 11, 1757.
Scientific background

Neuronal Pentraxin 1 (NP1 or NPTX1) is a 47–50 kDa secreted glycoprotein that is expressed in the central nervous system and involved in excitatory synaptic remodeling1

The pentraxin superfamily members are made from monomers arranged in pentameric structures with a discoid shape. The family members are characterized by a 205 amino acids long conserved sequence located at C-terminal called the pentraxin domain with a shorter inner conserved sequence called the pentraxin signature 2.

NP1 is preferentially expressed in the brain and is involved in glutamate receptor internalization. It is secreted pre-synaptically and implicated in excitatory synaptic and mitochondrial deficits. In addition, NP1 may represent a potential synapse-derived plasma biomarker that is able to identify alterations in excitatory synapses and early-onset Alzheimer's disease3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/04/2021

Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and  immunohistochemistry applications. It has been designed to recognize NPTX1 from rat, mouse and human samples.

For research purposes only, not for human use
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