Free shipping starts now, no minimum, no coupons required!

Anti-Neuropeptide S Receptor (extracellular) Antibody

NPSR1, G-Protein Coupled Receptor 154, GPR154, G-Protein Coupled Receptor for Asthma Susceptibility, GPRA

Cat #: ANR-029
Alternative Name NPSR1, G-Protein Coupled Receptor 154, GPR154, G-Protein Coupled Receptor for Asthma Susceptibility, GPRA
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DNFNVLPDTKERFYAS, corresponding to amino acid residues 297 - 312 of mouse NPSR1 (Accession Q8BZP8). Extracellular, 3rd loop.
Accession (Uniprot) Number Q8BZP8
Gene ID 319239
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – 15 out of 16 amino acid residues identical, Human – 14 out of 16 amino acid residues identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Neuropeptide S Receptor (extracellular) Blocking Peptide (#BLP-NR029)
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain lysate (lanes 1 and 5), rat brain lysate (lanes 2 and 6), rat small intestine lysate (lanes 3 and 7) and rat lung lysate (lanes 4 and 8):
    Western blot analysis of mouse brain lysate (lanes 1 and 5), rat brain lysate (lanes 2 and 6), rat small intestine lysate (lanes 3 and 7) and rat lung lysate (lanes 4 and 8):
    1-4. Anti-Neuropeptide S Receptor (extracellular) Antibody (#ANR-029), (1:200).
    5-8. Anti-Neuropeptide S Receptor (extracellular) Antibody, preincubated with Neuropeptide S Receptor (extracellular) Blocking Peptide (BLP-NR029).
  • Western blot analysis of human THP-1 monocytic leukemia cell line lysate:
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate:
    1. Anti-Neuropeptide S Receptor (extracellular) Antibody (#ANR-029), (1:200).
    2. Anti-Neuropeptide S Receptor (extracellular) Antibody, preincubated with Neuropeptide S Receptor (extracellular) Blocking Peptide (BLP-NR029).
Immunohistochemistry
  • Expression of Neuropeptide S Receptor in rat supraoptic nucleus (SON).
    Expression of Neuropeptide S Receptor in rat supraoptic nucleus (SON).
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuropeptide S Receptor (extracellular) Antibody (#ANR-029), (1:400), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. NPSR1 immunoreactivity (red) appears in SON neurons (arrows). B. Pre-incubation of the antibody with Neuropeptide S Receptor (extracellular) Blocking Peptide (BLP-NR029), suppressed staining. Cell nuclei are stained with DAPI (blue). OT = optic tract, SON = supraoptic nucleus.
Indirect flow cytometry
  • Cell surface detection of NPSR1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of NPSR1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Neuropeptide S Receptor (extracellular) Antibody (#ANR-029), (5μg) + goat-anti-rabbit-FITC.
References
  1. Valsalan et al. (2014) Gen Comp Endocrinol, 1, 209.
  2. Zhang, Z. R. et al. (2019) Prog. Mol. Biol. Transl. Sci. 161, 125–148.
  3. Ruzza C et al. (2015) Neuropharmacology, 97,1-6.
  4. Duangdao DM et al. (2009) Behav Brain Res, 14, 205.
  5. Holanda VAD et al.  (2021) Pharmaceuticals (Basel), 6, 14.
Scientific background

The Neuropeptide S Receptor (NPSR1), also known as GPR154, GPRA, or VRR1, belongs to the G protein-coupled receptor (GPCR) superfamily and is activated by neuropeptide S (NPS)1,2.

NPS is a 20-residue peptide, named due to the presence of a serine (S) residue in the N-terminal position of the molecule. NPS expression is limited to a few discrete brain areas, such as in isolated cells of the amygdala, the hypothalamus, and specific regions of the brainstem. The primary sequence of NPS is highly conserved among vertebrates especially at the N-terminus, which is crucial for biological activity. The positions 2, 3, 4, and 5 on N-terminus of NPS play important roles in bioactivity and activation of NPSR11,2.

The Neuropeptide S Receptor is widely distributed in the brain particularly in regions that are associated with regulation of stress response, memory, the olfactory system, and regulation of arousal.  NPSR1 can signal via both Gq and Gs to increase cellular excitability and inhibit cell growth. Via the Gq pathway, NPSR1 leads to activation of Gq–phospholipase C and cleavage of phospholipid phosphatidylinositol 4,5-bisphosphate to inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. IP3 activates IP3 receptor and releases Ca2 + from the ER. In some neurons, store-operated calcium channels are also activated, allowing entry of extracellular calcium into the cells 2.

In the Gs pathway, NPSR signaling activates adenylate cyclase, increased synthesis of cAMP, and activation of protein kinase A 2.

NPSR1 activation, like other GPCRs, also leads to activation of extracellular signal-regulated kinase 1/2 (ERK1/2) 2

Knockout studies in mice demonstrated that the NPS system produces anti-aggressive effects, reduces social anxiety behavior, modulates fear response and enhances motor performance skills3,4

A growing body of evidence suggests a close relationship between neuropeptide S and its receptor NPSR1 system in Parkinson's disease (PD). Indeed, administration of NPS to the central nervous syatem, ameliorates motor and non-motor dysfunctions in PD models, suggesting that targeting the NPS/NPSR1 system might be beneficial for Parkinson-related signs and symptoms 5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Anti-Neuropeptide S Receptor (extracellular) Antibody (#ANR-029) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize NPSR1 from mouse, rat and human samples.

For research purposes only, not for human use
Shipping and Ordering information