- Peptide SFIRKHRRRYSKKTAC, corresponding to amino acid residues 291-306 of rat NPY5R (Accession Q63634). 3rd intracellular loop.
- Western blot analysis of rat brain membrane:1. Anti-NPY5R Antibody (#ANR-025), (1:200).
2. Anti-NPY5R Antibody, preincubated with NPY5R Blocking Peptide (#BLP-NR025).
- Expression of neuropeptide Y5 receptor in rat striatumImmunohistochemical staining of rat striatum using Anti-NPY5R Antibody (#ANR-025). A. NPY5R (red) appears in the striatal matrix (asterisk) and in nerve cells in the matrix (vertical arrows). B. Parvalbumin (green) appears in the striatal matrix. C. Merge of NPY5R and parvalbumin demonstrates co-localization in a sub-set of cells (horizontal arrows). DAPI is used as the counterstain (blue).
- Expression of neuropeptide Y5 receptor in rat DRGImmunocytochemical staining of a primary culture of rat dorsal root ganglion (DRG) neurons. A, D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY5R Antibody (#ANR-025), (1:100) followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images of panels D and E.
Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter present in the central and peripheral nervous systems.1 NPY is involved in the regulation of a broad range of functions such as eating behavior, hypertension, and reproduction.2
NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.
Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase signalling, and a number of ion channel functions.3 Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2
NPY5R as well as NPY1R and NPY2R are expressed in the hypothalamus, in the brain stem, and in peripheral tissues, such as blood vessels, lung, kidney, adrenal glands, stomach, colon, heart, pancreas, and intestine. NPY5R has been postulated as the mediator of the feeding response to NPY and related peptides (125 in Kamiji, M. M). Its localization in the brain is consistent with this hypothesis.4
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat neuropeptide Y5 receptor (NPY5R). Anti-NPY5R Antibody (#ANR-025) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NPY5R from rat, human and mouse samples.