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Anti-NGL-2/LRRC4 (extracellular) Antibody

Netrin-G2 Ligand, Brain Tumor-Associated Protein BAG, Leucine-Rich Repeat-Containing Protein 4

Cat #: ANR-162
Alternative Name Netrin-G2 Ligand, Brain Tumor-Associated Protein BAG, Leucine-Rich Repeat-Containing Protein 4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)STDTTDKMQTSLDE, corresponding to amino acid residues 507 - 520 of mouse LRRC4 (Accession Q99PH1). Extracellular, N-terminus.
Accession (Uniprot) Number Q99PH1
Gene ID 192198
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical, Human 13 out of 14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain synaptosome preparation (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain synaptosome preparation (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1-2. Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200).
    3-4. Anti-NGL-2/LRRC4 (extracellular) Antibody, preincubated with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysates:
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysates:
    1. Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200).
    2. Anti-NGL-2/LRRC4 (extracellular) Antibody, preincubated with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162).
Immunohistochemistry
  • Expression of NGL-2/LRRC4 in rat hypothalamus.
    Expression of NGL-2/LRRC4 in rat hypothalamus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. NGL-2/LRRC4 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third cerebral ventricle
  • Expression of NGL-2/LRRC4 in mouse cortex.
    Expression of NGL-2/LRRC4 in mouse cortex.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. NGL-2/LRRC4 immunoreactivity (green) appears in cortical neurons (arrows). B. Pre-incubation of the antibody with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Ko, J. (2012) Mol. Cells., 31, 335.
  2. Zhang, Q. et al. (2005) FEBS. Lett., 4, 3674.
  3. Li, P. et al. (2014) Mol. Cancer., 19, 1476.
  4. Um, S.M. et al. (2018) Cell. Rep., 26, 3839.
  5. Choi, Y. et al. (2019) Front. Mol. Neurosci., 12, 119.
Scientific background

The leucine-rich repeat (LRR) superfamily is a diverse protein family that consist of leucine-rich domains (LRR domains). The LRR domain is known to regulate protein-protein interaction or cell adhesion. Large portion of LRR proteins mediate the differentiation and development of nervous tissues1.

One member of the LRR superfamily is leucine rich repeat containing protein 4 (LRRC4), also known as netrin-G ligand-2 (NGL-2), and serves as a receptor for netrin-G2 (NTNG2). In addition to the LRR domain LRRC4 consist of immunoglobulin (Ig) domain in the extracellular region, a transmembrane domain, and a cytoplasmic region that terminates in a protein-protein interaction domain (PDZ)-binding motif. LRRC4 regulates the formation of excitatory synapses and promotes axon differentiation2.

Functionally, deletion of LRRC4 in mice was found to suppress excitatory synapse development and synaptic transmission. In addition, LRRC4 gene has been identified as tumor suppressor3. In human it has been implicated in autism spectrum disorder (ASD), schizophrenia, and intellectual disability. Moreover, netrin-G2, the presynaptic ligand of LRRC4, is linked to schizophrenia and bipolar disorder4-5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/01/2022

Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize LRRC4 from rat, mouse and human samples.

For research purposes only, not for human use
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