Anti-NGL-3/LRRC4B (extracellular) Antibody

Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4
    Cat #: ANR-163
    Alternative Name Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    • Peptide (C)PGEEAQQPRGTEKE, corresponding to amino acid residues 498-511 of mouse NGL-3 (Accession P0C192). Extracellular, N-terminus.
    • Anti-NGL-3/LRRC4B (extracellular) Antibody
    Accession (Uniprot) Number P0C192
    Gene ID 272381
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – identical; human – 13 out of 14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-NGL-3/LRRC4B (extracellular) Antibody
      Western blot analysis of rat new born brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
      1,2. Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200).
      3,4. Anti-NGL-3/LRRC4B (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-NGL-3/LRRC4B (extracellular) Antibody
      Western blot analysis of mouse brain lysate:
      1. Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200).
      2. Anti-NGL-3/LRRC4B (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-NGL-3/LRRC4B (extracellular) Antibody
      Expression of NGL-3 in mouse hippocampus
      Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200), followed by goat anti-rabbit-AlexaFluor-488. NGL-3 immunoreactivity (green) is detected in the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
    1. Seiradake, E. et al. (2011) EMBO J. 30, 4479.
    2. Kwon, S.K. et al. (2010) Biol. Chem. 285, 13966.
    3. Lin, J.C. et al. (2003) Neurosci. 6, 1270.
    4. Kim, S. et al. (2006) Neurosci. 9, 1294.
    5. Woo, J. et al. (2009) Neurosci. 12, 428.
    6. Lee, H. et al. (2019) PLoS Biol. 17, e2005326.
    Scientific background

    NGL-3 (Netrin G ligand 3) is a postsynaptic cell adhesion molecule that is a member of the NGL family. NGLs are proteins involved in the regulation of various steps in synapse formation. Members of this family share a common domain structure that contains: nine Leucine rich repeats (LRRs), an immunoglobulin (Ig) domain in the extracellular region, a single trans membrane domain and a cytoplasmic region that ends with a PDZ domain-binding motif. This motif mediates the interaction with PSD-95, an abundant postsynaptic scaffolding protein1,2.

    The NGL family is comprised of three proteins: NGL-1, 2, and 3. NGL-1 and NGL-2 interact through their extracellular domain with the GPI anchored membrane proteins Netrin G1 and G2 respectively2-4. This interaction is important for regulation of structural and functional excitatory synapse development4.

    NGL-3 trans-synaptically interacts with the leukocyte common antigen-related (LAR) protein which is well-known for its involvement in axon guidance and presynaptic differentiation. This interaction induces pre- and postsynaptic differentiation in contacting axons and dendrites, respectively, and regulates excitatory synapse formation2,5.

    Mice lacking NGL-3 (Ngl3−/−) show markedly suppressed normal brain development and postnatal survival and growth6. NGL-3 overexpression and knockdown in cultured neurons result in bidirectional changes in the number of excitatory synapses and spontaneous excitatory synaptic transmission5.

    NGL-3 mRNA and proteins are mainly detected in synaptic fractions of the brain and levels gradually increase during the first 3 weeks of postnatal rat brain development5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 27/01/2020

    Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize LRRC4B from rat, mouse, and human samples.

    For research purposes only, not for human use



    Scientific Background


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