Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody

nAChR β2, Neuronal acetylcholine receptor subunit beta-2, Cholinergic receptor nicotinic beta 2, ACRB2, EFNL3
    Cat #: ANC-012
    Alternative Name nAChR β2, Neuronal acetylcholine receptor subunit beta-2, Cholinergic receptor nicotinic beta 2, ACRB2, EFNL3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)EDFDNMKKVRLP, corresponding to amino acid residues 96-107 of rat nAChRβ2 (Accession P12390). Extracellular, N-terminus.
        Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody
    Accession (Uniprot) Number P12390
    Gene ID 54239
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 11/12 amino acid residues identical.
    RRID AB_10917442.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
      • Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody
        Western blot analysis of SH-SY5Y human neuroblastoma cell lysate (lanes 1 and 4) and rat (lanes 2 and 5) and mouse (lanes 3 and 6) brain lysate:
        1-3. Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody (#ANC-012), (1:200).
        4-6. Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody, preincubated with the negative control antigen.
      • Mouse pancreas sections (1:200) (Ganic, E. et al. (2016) Cell Rep. 14, 1991.).
      • Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody
        Expression of nAChR β2 in rat PC12 cells
        Cell surface detection of nAChR β2 in live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody (#ANC-012), (1:100), followed by goat anti-rabbit-AlexaFluor- 594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Live view of the cells.
    References
      • Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs), which belong to the G-protein coupled receptor (GPCR) superfamily, and nicotinic AChRs (nAChRs), which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1.

        To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, three conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1.

        While most β subunits are neuronal, the β1 subunit forms functional receptors along with other subunits in the muscle3. The diversity of these receptors and their functional organization gives rise to unique properties and functions. The α4β2 receptor composition makes up a high affinity nicotinic receptor. In fact, its upregulation (mainly expressed by the increase of functional receptors at the membrane and not expression per se) is responsible for the increased appearance of binding sites following nicotine administration1,3.

        Animal studies have shown that nAChR-related mechanisms are involved in attention function4. Indeed α4β2 nAChR seems to also be involved in attention-deficit hyperactivity disorder (ADHD), a disease distinguished by a lack of attention, distractibility and hyperactivity3. The α4β2 and α7 nAChRs appear to be critical in rats for attention and working memory. Also, a α4β2 specific agonist was shown to reduce impulsivity, hyperactivity and attention deficits in adults with ADHD5. This same receptor subtype may also be involved in Parkinson’s disease (PD) as smoking and α4β2 nAChR agonists show beneficial effects in PD6,7.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 11/11/2019

    Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody (#ANC-012) is a highly specific antibody directed against an epitope the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize nAChRβ2 from mouse, rat, and human samples.

    For research purposes only, not for human use
    Citations
      • Mouse pancreas lysate (1:500).
        Ganic, E. et al. (2016) Cell Rep. 14, 1991.
      • Mouse pancreas sections (1:200).
        Ganic, E. et al. (2016) Cell Rep. 14, 1991.
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