Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody

nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4
    Cat #: ANC-014
    Alternative Name nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide CYEGVNILRIPAKR, corresponding to amino acid residues 95-108 of rat nAChRβ4 (Accession P12392). Extracellular, N-terminus.
        Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody
    Accession (Uniprot) Number P12392
    Gene ID 25103
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 13/14 amino acid residues identical.
    RRID AB_11218956.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
      • Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody
        Western blot analysis of SH-SY5Y human neuroblastoma cell lysate (lanes 1 and 4), rat dorsal root ganglion (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:
        1-3. Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody (#ANC-014), (1:200).
        4-6. Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody
        Expression of nAChR β4 in rat dorsal root ganglion
        Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody (#ANC-014). A. nAChRβ4 labeling (red) appears in neuronal cell bodies. B. Nuclear staining using DAPI as counterstain (blue). C. Merged images of A and B.
      • Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody
        Expression of nAChR β4 in rat PC12 cells
        Cell surface detection of nAChR β4 in live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody (#ANC-014), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
    References
      • Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs), which belong to the G-protein coupled receptor (GPCR) superfamily, and nicotinic AChRs (nAChRs), which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1.

        To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which are responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, three conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1.

        While most β subunits are neuronal, the β1 subunit forms functional receptors along with other subunits in the muscle3. In neurons the α2-α6 and β2-β4 subunits form heteropentameric receptors, usually with a (αx)2y)3 stoichiometry3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 11/11/2019

    Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody (#ANC-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize nAChRβ4 from mouse, rat, and human samples.

    For research purposes only, not for human use
    Citations
      • Mouse pancreas lysate (1:500).
        Ganic, E. et al. (2016) Cell Rep. 14, 1991.
      • Mouse pancreas sections (1:200).
        Ganic, E. et al. (2016) Cell Rep. 14, 1991.
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