Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO-594 Antibody

nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4
    Cat #: ANC-014-AR
    Alternative Name nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CYEGVNILRIPAKR, corresponding to amino acid residues 95-108 of rat nAChRβ4 (Accession P12392). Extracellular, N-terminus.
    • Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO-594 Antibody
    Accession (Uniprot) Number P12392
    Gene ID 25103
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 13/14 amino acid residues identical.
    RRID AB_2341002.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANC-014), and immunohistochemistry (labeled antibody).
    Applications: ic, if, ih, lci
    Immunohistochemistry
    • Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO-594 Antibody
      Expression of nAChR β4 in rat DRG
      Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO-594 Antibody (#ANC-014-AR), (1:100). A. nAChRβ4 labeling (red) appears in neuronal cell bodies. B. Nuclear staining using DAPI as counterstain (blue). C. Merged images of A and B.
    Live cell imaging / Immunocytochemistry
    • Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO-594 Antibody
      Expression of nAChR β4 in human SH-SY5Y cells
      Cell surface detection of nAChR β4 in live intact human SH-SY5Y neuroblastoma cells. A. Cells were stained with Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO-594 Antibody (#ANC-014-AR), (1:100), (red). B. Live image of the cells. C. Merge of the two images.
    References
    1. Albuquerque, E.X. et al. (2009) Physiol. Rev. 89, 73.
    2. Karlin, A. et al. (1986) Ann. NY. Acad. Sci. 463, 53.
    3. Kalamida, D. et al. (2007) FEBS J. 274, 3799.
    Scientific background

    Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs), which belong to the G-protein coupled receptor (GPCR) superfamily, and nicotinic AChRs (nAChRs), which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1.

    To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which are responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, three conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1.

    While most β subunits are neuronal, the β1 subunit forms functional receptors along with other subunits in the muscle3. In neurons the α2-α6 and β2-β4 subunits form heteropentameric receptors, usually with a (αx)2y)3 stoichiometry3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody (#ANC-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It and has been designed to recognize nAChRβ4 from mouse, rat, and human samples.

    Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO-594 Antibody (#ANC-014-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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