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Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody

nAChR α9, nAChR alpha-9, Neuronal acetylcholine receptor subunit alpha-9, ACRA9

Cat #: ANC-019
Alternative Name nAChR α9, nAChR alpha-9, Neuronal acetylcholine receptor subunit alpha-9, ACRA9
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KDHKATNSKGSEWKK, corresponding to amino acid residues 436-450 of rat nAChRα9 (Accession P43144). 2nd intracellular loop.
Accession (Uniprot) Number P43144
Gene ID 65024
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756678.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody (#ANC-019), (1:400).
    3,4. Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody, preincubated with Nicotinic Acetylcholine Receptor α9/CHRNA9 Blocking Peptide (#BLP-NC019).
Immunohistochemistry
  • Expression of nAChRα9 in rat DRG
    Expression of nAChRα9 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody (#ANC-019), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. nAChRα9 staining (in green) appears in neuronal soma (arrows). DAPI is used as the counterstain (blue).
References
  1. Lustig, L.R. et al. (2006) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 288, 424.
  2. McIntosh, J.M. et al. (2009) Biochem. Pharmacol. 78, 693.
  3. Filchakova, O. et al. (2013) PLoS One. 8, e64655.
Scientific background

The nicotinic acetylcholine receptors (nAChRs) are ionotropic multi-subunit, neurotransmitter-gated receptors of the cholinergic system. These receptors are responsible for mediating the effects of the neurotransmitter acetylcholine (ACh). They are assembled from one or more α subunits (α1-α10) alone or together with one or more β subunits (β1–β4).

The receptors are also a target of the biologic compound nicotine, which mostly mimics the effects of acetylcholine on the receptors by binding as an agonist to α subunit of nAChRs.

nAChRs play critical physiologic roles in the central and peripheral nervous systems. They regulate neurotransmitter release, cell excitability, neuronal integration, and are involved in functions such as sleep and arousal patterns, fatigue, hunger, anxiety, and pain processing1,2.

The structure of each subunit is composed of an extracellular domain, which harbors the binding site, four transmembrane α-helices (TM1–4), and a variable intracellular region4. The α subunits have a defining “cysteine loop” that contains two vicinal cysteine residues3.

nAChRs containing the α9 subunit are expressed in a variety of non-neuronal tissues starting from immune cells to breast carcinomas. The α9 subunit is able to form a functional homomeric receptor and in addition to co-assemble with the α10 subunit into functional heteromeric nAChRs3.

α9-containing nAChRs play an important role in pain, inflammation, keratinocyte adhesion and in mediating synaptic transmission between the efferent olivocochlear fibers and cochlear hair cells3.

nAChRs are involved in pathologies, such as myasthenia, epilepsy, schizophrenia, Parkinson’s disease, autism, dementia with Lewy bodies, Alzheimer’s disease, and addiction3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 25/07/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat nAChRα9. Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody (#ANC-019) can be used western blot and immunohistochemistry applications. It has been designed to recognize nAChRα9 from human, rat and mouse samples.

For research purposes only, not for human use
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