Free shipping starts now, no minimum, no coupons required!

Accessibility

Accessibility Statement

 
Introduction
The internet today constitutes the largest database of free information for all users, including users with disabilities. As such, we place great emphasis on providing equal opportunity for people with disabilities to be able to use the information on our website and enjoy a better browsing experience. 
We aim to ensure that digital services are accessible to people with disabilities, and therefore we have invested large resources in order to simplify the use of the website for people with disabilities as much as possible, out of the belief that every person deserves the right to live with equality, dignity, convenience, and independence. 
 
Using the website accessibility program
This website is run by the accessibility program of the "Accessible with a Click" company and is run via a designated accessibility server. The program allows the website to follow the guidelines for internet content accessibility WCAG 2.0 to level AA. The program is subject to the conditions of use of the manufacturer. Warrantee of use applies to the website owners and/or their representative, including the content displayed in the website, as subject to the conditions of use. 
 
How does accessibility work on the website?

The website has an accessibility menu. Clicking on the menu opens accessibility buttons. After choosing a subject in the menu, waiting time is necessary for the page to upload. 

What is in the menu?

 

✓ Ability to navigate with the keyboard
✓ Increasing and decreasing the website fonts
✓ Changing color contrast based on dark backgrounds
✓ Changing color contrast based on light backgrounds
✓ Adapting the website to color blind people
✓ Underlining links throughout the website

 

Clarification

Despite our efforts to enable website browsing for all the website pages, there may be website pages that haven't been made accessible yet or may lack a suitable technical solution.
We are continuing our efforts to enhance the accessibility of the website as much as possible, out of our moral obligation to enable the use of the website for the population as a whole, including people with disabilities. 
Alomone Labs Alomone Labs

Order Summary

Home

main

    • column
      • Ion Channels
    • column
      • G-Protein Coupled Receptors
      • Transporters, Exchangers and Pumps
    • column
      • Neurotrophic Factors and Receptors
      • Neural Signaling
    • column
      • Cell Imaging Tools
      • Research Areas
  • Home
  • Products
    • column
      • New Products
      • All Products
      • Research Areas
      • Select Targets
    • column
      • Ion Channels
      • G-Protein Coupled Receptors
      • Transporters, Exchangers and Pumps
      • Controls
    • column
      • Neurotrophic Factors and Receptors
      • Neural Signaling
      • Cell Imaging Tools
      • Kits & Packs
    • column
      • Guinea Pig Polyclonal Antibodies
      • Multiplex Staining Products
      • Live Cell Imaging Products
      • Live Cell Flow Cytometry Products
  • Services
    • column
      • Custom Services
      • Online Quotes New
      • Ordering & Shipping
  • Resources
    • column
      • Protocols
      • FAQs and Troubleshooting
      • Citations Search Tool
    • column
      • Blog
      • Articles
      • Download Protocol Book
  • About
    • column
      • About Alomone Labs
      • Why Alomone Labs?
  • Contact
    • column
      • Contact Us
      • Subscribe – Newsletters and Email Updates
      • Subscribe – Printed Magazines
Return to Content
Home › Products › Ion Channels › Ligand-Gated Ion Channels › Glutamate Receptors › NMDA Receptors › Antibodies to NMDA Receptors

Certificate of Analysis

Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody

NMDA receptor 2A, NR2A, GRIN2A, Ionotropic glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A

Cat #: AGC-002-AG
Alternative Name NMDA receptor 2A, NR2A, GRIN2A, Ionotropic glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide GHSHDVTERELRN(C), corresponding to amino acid residues 41-53 of rat NR2A (Accession Q00959). Extracellular, N-terminus.
Accession (Uniprot) Number Q00959
Gene ID 24409
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, mouse - identical.
RRID AB_2756596.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody,#AGC-002), and immunohistochemistry (labeled antibody).
Applications: if, ih
May also work in: ic*, lci*
Immunohistochemistry
  • Mouse brain sections (frozen), (1:80).
  • Expression of NR2A in mouse brain sections
    Expression of NR2A in mouse brain sections
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody (#AGC-002-AG), (1:80). A. NR2A staining (green) in striatum is detected in neuronal profiles. B. NR2A staining in cingulate cortex is also detected in neuronal profiles. Cell nuclei are labeled with DAPI (blue).
Scientific background

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.

The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.

NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.

Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s disease.

NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Back to product pageSDSNew Generate a Quote
Image & Title:

Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 AntibodyMultiplex staining of GluN2A and SynGAP in rat cingulate cortex.Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody (#AGC-002-AG), (1:200) and Anti-SynGAP Antibody (#APZ-032), (1:200), followed by donkey-anti-rabbit-Cy3. A. GluN2A staining (green) appears in neuronal profiles (arrows). B. SynGAP staining (red) is detected mostly in apical dendrites (horizontal arrows) and in neuronal soma (vertical arrows). C. Merge of the two images demonstrates colocalization in some neurons (vertical arrows). Cell nuclei are stained with DAPI (blue).

Last update: 08/01/2023

See Related Products

For research purposes only, not for human use

Specifications

Citations

Citations

Applications

Scientific Background

Shipping and Ordering information
    Back to product pageSDSNew Generate a Quote

    Control Product

    • NMDAR2A/GluN2A (extracellular) Blocking Peptide (#BLP-GC002) is the original antigen used for immunization during Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002) generation. The blocking peptide binds and ‘blocks’ Anti-NMDAR2A/GluN2A (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

      NMDAR2A/GluN2A (extracellular) Blocking Peptide (#BLP-GC002)

    Resources

    • ATTO Fluorescent Dyes

    Free International Shipping

    Products are lyophilized and ship at room temperature - FREE OF CHARGE.

    We ship worldwide within 24 hours of our next business day.

    For more country-specific shipping and contact information see Ordering & Shipping.


    Free Sample Program

    Not sure whether the product fits your experimental setup?

    Try it first!


    Alomone Guarantee

    We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

    Alomone Guarantee information

    Resources

    • ATTO Fluorescent Dyes

    Need Help With This Product?

    Our reagent specialists are here to help you find the best product for your application. Please call or email us and we will be happy to help you find the right product for the job.

    Call us at
    +972 2587 2202 or Email Us

    Contact Us

    © 2023 Alomone Labs. All Rights Reserved.

    Terms of Service Privacy Policy
    Scroll to top

    Bulk / Customized order