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Anti-NMUR1 (extracellular) Antibody

Neuromedin-U Receptor 1, G Protein-Coupled Receptor 66, GPR66

Cat #: ANR-071
Alternative Name Neuromedin-U Receptor 1, G Protein-Coupled Receptor 66, GPR66
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DRLMWSMVSHWTDGLR, corresponding to amino acid residues 316 - 331 of rat NMUR1 (Accession Q9JJI5). Extracellular, 3rd loop.
Accession (Uniprot) Number Q9JJI5
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human – 12 out of 16 amino acid residues identical;
Mouse - 10 out of 16 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1-2. Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1:500).
    3-4. Anti-NMUR1 (extracellular) Antibody, preincubated with NMUR1 (extracellular) Blocking Peptide (BLP-NR071).
  • Western blot analysis of human HT-29 colon adenocarcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human K562 myelogenous leukemia cell line lysate (lanes 3 and 7) and human NK-92 natural killer cell line lysate (lanes 4 and 8):
    Western blot analysis of human HT-29 colon adenocarcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human K562 myelogenous leukemia cell line lysate (lanes 3 and 7) and human NK-92 natural killer cell line lysate (lanes 4 and 8):
    1-4. Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1:400).
    5-8. Anti-NMUR1 (extracellular) Antibody, preincubated with NMUR1 (extracellular) Blocking Peptide (BLP-NR071).
Immunohistochemistry
  • Expression of NMUR1 in rat cerebellum.
    Expression of NMUR1 in rat cerebellum.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1: 300), followed by goat anti-rabbit-AlexaFluor-488. A. NMUR1 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with NMUR1 (extracellular) Blocking Peptide (BLP-NR071), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of NMUR1 by indirect flow cytometry in live intact mouse J774 macrophage cell line:
    Cell surface detection of NMUR1 by indirect flow cytometry in live intact mouse J774 macrophage cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-NMUR1 (extracellular) Antibody (#ANR-071), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Brighton, P.J. et al. (2004) Pharmacol. Rev. 56, 231.
  2. Panetta, R. et al (2013) Physiol Genomics. 45, 89.
Scientific background

Neuromedin U Receptor 1 (NMUR1) is one of the two receptors for the neuropeptide Neuromedin U (NMU). NMUR1 and the second receptor NMUR2 are members of the G protein coupled receptors (GPCRs) superfamily 1.

NMU is a neuropeptide with potent activity on smooth muscles that is widely distributed in the gut and central nervous system. NMU interaction with one of its two receptors- NMU1R or NMU2R, mediates the stimulation of smooth muscles, increase of blood pressure, alteration of ion transport in the gut, control of local blood flow and regulation of adrenocortical function1-2.

NMU1R is abundantly expressed in peripheral tissues including lung, GI tract, pancreatic islets, testis, adipose, immune tissues, and dorsal root ganglia2, and due to its diverse functions it is a very appealing drug and research candidate.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-NMUR1 (extracellular) Antibody (#ANR-071) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize NMUR1 from rat, mouse and human samples

For research purposes only, not for human use
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