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Anti-Nogo Receptor (extracellular)-FITC Antibody

NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R

Cat #: ANT-008-F
Alternative Name NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo Receptor (Accession Q9BZR6). Extracellular.
Accession (Uniprot) Number Q9BZR6
Gene ID 65078
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat - 15/16 amino acid residues identical.
RRID AB_2876822
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANT-008), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection of Nogo Receptor in live intact mouse BV-2 microglia cells:
    Cell surface detection of Nogo Receptor in live intact mouse BV-2 microglia cells:
    ___ Cells.  
    ___ Cells + rabbit IgG isotype control-FITC.  
    ___ Cells + Anti-Nogo Receptor (extracellular)-FITC Antibody (#ANT-008-F), (2.5 µg).
  • Cell surface detection of Nogo Receptor in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of Nogo Receptor in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.  
    ___ Cells + rabbit IgG isotype control-FITC.  
    ___ Cells + Anti-Nogo Receptor (extracellular)-FITC Antibody (#ANT-008-F), (2.5 µg).
References
  1. Kim, M.J. et al. (2018) Cells 8, 1.
  2. Fournier, A.E. et al. (2001) Nature 409, 341.
  3. Lee, H. et al. (20018) J. Neurosci. 28, 2753.
  4. Teng, F.Y.H. and Tang, B.L. (2005) Neurosci. Res. 79, 273.
Scientific background

Neurite outgrowth inhibitor receptor 1 (NgR1, Nogo Receptor) is a glycosylphosphatidylinositol (GPI) transmembrane protein expressed on the surface of axons in the CNS. Its key binding domain is an extracellular leucine-rich repeat domain that interacts with Myelin-associated inhibitory factors (MAIFs) composed of MAG, Neurite outgrowth inhibitor (Nogo)-A, and OMgp1,2.

NgR1 binds to Neurite outgrowth inhibitor (Nogo) through a short hydrophilic loop between two hydrophobic domains. This binding activates intracellular molecular switches through the heteromerization with NgR1 co-receptors, p75NTR and LINGO-1.

This process facilitates neurite outgrowth dynamics; it also governs the plasticity of the CNS at the axo-dendritic and axo-glial levels3.

During CNS neurodegenerative disorders and in animal models of inflammatory demyelination, MAIFs, containing Nogo, accumulate and therefore there is an increased binding to their cognate receptor. This marks Nogo-NgR1 interaction as a potential target of new therapeutics for neurodegenerative diseases4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Nogo Receptor (extracellular) Antibody (#ANT-008) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NgR1 from human, mouse and rat samples.

Anti-Nogo Receptor (extracellular)-FITC Antibody (#ANT-008-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

For research purposes only, not for human use
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