Anti-Nogo Receptor (extracellular) Antibody

NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R
    Cat #: ANT-008
    Alternative Name NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor (Accession Q9BZR6). Extracellular.
    • Anti-Nogo Receptor (extracellular) Antibody
    Accession (Uniprot) Number Q9BZR6
    Gene ID 65078
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat - 15/16 amino acid residues identical.
    RRID AB_2040180.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-Nogo Receptor (extracellular) Antibody
      Western blot analysis of rat brain lysate:
      1. Anti-Nogo Receptor (extracellular) Antibody (#ANT-008), (1:200).
      2. Anti-Nogo Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    • Anti-Nogo Receptor (extracellular) Antibody
      Expression of Nogo receptor in rat cerebellum
      Immunohistochemical staining of rat cerebellum using Anti-Nogo Receptor (extracellular) Antibody (#ANT-008). A. NgR (red) appears in Purkinje cells (arrows). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of NgR and calbindin D28K demonstrates some co-localization of these proteins.
    Live cell imaging / Immunocytochemistry
    • Anti-Nogo Receptor (extracellular) Antibody
      Expression of Nogo receptor in rat cerebellar granule
      Cell surface detection of Nogo receptor in live cultured rat cerebellar granule. A. Cells were stained with Anti-Nogo Receptor (extracellular) Antibody (#ANT-008) followed by goat-anti-rabbit AlexaFluor-555 secondary antibody (red). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue). B. Live view of the same field as in A.
    References
    1. Fournier, A.E. et al. (2001) Nature 409, 341.
    2. Prinjha, R. et al. (2000) Nature 403, 383.
    3. GrandPré, T. et al. (2000) Nature 403, 439.
    4. Domeniconi, M. et al. (2002) Neuron 35, 283.
    5. Wang, K.C. et al. (2002) Nature 417, 941.
    6. Wang, K.C. et al. (2002) Nature 420, 74.
    7. Mi, S. et al. (2004) Nat. Neurosci. 7, 221.
    Scientific background

    The Nogo receptor is a leucine-rich repeat (LRR) containing protein with a glycosylphosphatidylinositol (GPI) anchored C-terminus. The receptor was identified on the basis of its ability to bind with high affinity to Nogo-A a member of the reticulon family expressed in the cell membrane of oligodendrocytes.1

    Nogo-A attracted attention when it was demonstrated that it is a myelin protein capable of inhibiting axonal growth following nerve injury.2,3  Other myelin proteins also identified as axonal growth inhibitors are the myelin-associated glycoprotein (MAG) a sialic-dependent immunoglobulin-like family member lectin (SIGLEC) and the oligodendrocyte myelin glycoprotein (OmgP) which is a GPI-anchored membrane protein.  Remarkably, despite their structural diversity, all three axonal growth inhibitors bind to the Nogo receptor with high affinity.4,5

    Since the Nogo receptor is a GPI-anchored protein it was expected that it would require another protein component to transduce the Nogo-A binding information into the responding neurons interior. Indeed, it was shown that the neurotrophin receptor p75 (p75NTR) interacts with Nogo receptor and a third protein termed LINGO-1 to mediate axonal growth inhibition signaling.6,7

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human Nogo receptor. Anti-Nogo Receptor (extracellular) Antibody (#ANT-008) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NgR from human, mouse and rat samples.

    For research purposes only, not for human use
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