Anti-Nogo Receptor (extracellular) Antibody

NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R

Cat #: ANT-008
Alternative Name NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor (Accession Q9BZR6). Extracellular.
Accession (Uniprot) Number Q9BZR6
Gene ID 65078
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat - 15/16 amino acid residues identical.
RRID AB_2040180.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, lci, wb
May also work in: ip*
Western blot
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-Nogo Receptor (extracellular) Antibody (#ANT-008), (1:200).
    2. Anti-Nogo Receptor (extracellular) Antibody, preincubated with Nogo Receptor (extracellular) Blocking Peptide (#BLP-NT008).
Immunohistochemistry
  • Expression of Nogo receptor in rat cerebellum
    Expression of Nogo receptor in rat cerebellum
    Immunohistochemical staining of rat cerebellum using Anti-Nogo Receptor (extracellular) Antibody (#ANT-008). A. NgR (red) appears in Purkinje cells (arrows). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of NgR and calbindin D28K demonstrates some co-localization of these proteins.
Indirect flow cytometry
  • Cell surface detection of Nogo Receptor in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of Nogo Receptor in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Nogo Receptor (extracellular) Antibody (#ANT-008), (2.5 µg) + goat-anti-rabbit-FITC.
Live cell imaging / Immunocytochemistry
  • Expression of Nogo receptor in rat cerebellar granule
    Expression of Nogo receptor in rat cerebellar granule
    Cell surface detection of Nogo receptor in live cultured rat cerebellar granule. A. Cells were stained with Anti-Nogo Receptor (extracellular) Antibody (#ANT-008) followed by goat-anti-rabbit AlexaFluor-555 secondary antibody (red). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue). B. Live view of the same field as in A.
References
  1. Fournier, A.E. et al. (2001) Nature 409, 341.
  2. Prinjha, R. et al. (2000) Nature 403, 383.
  3. GrandPré, T. et al. (2000) Nature 403, 439.
  4. Domeniconi, M. et al. (2002) Neuron 35, 283.
  5. Wang, K.C. et al. (2002) Nature 417, 941.
  6. Wang, K.C. et al. (2002) Nature 420, 74.
  7. Mi, S. et al. (2004) Nat. Neurosci. 7, 221.
Scientific background

The Nogo receptor is a leucine-rich repeat (LRR) containing protein with a glycosylphosphatidylinositol (GPI) anchored C-terminus. The receptor was identified on the basis of its ability to bind with high affinity to Nogo-A a member of the reticulon family expressed in the cell membrane of oligodendrocytes.1

Nogo-A attracted attention when it was demonstrated that it is a myelin protein capable of inhibiting axonal growth following nerve injury.2,3  Other myelin proteins also identified as axonal growth inhibitors are the myelin-associated glycoprotein (MAG) a sialic-dependent immunoglobulin-like family member lectin (SIGLEC) and the oligodendrocyte myelin glycoprotein (OmgP) which is a GPI-anchored membrane protein.  Remarkably, despite their structural diversity, all three axonal growth inhibitors bind to the Nogo receptor with high affinity.4,5

Since the Nogo receptor is a GPI-anchored protein it was expected that it would require another protein component to transduce the Nogo-A binding information into the responding neurons interior. Indeed, it was shown that the neurotrophin receptor p75 (p75NTR) interacts with Nogo receptor and a third protein termed LINGO-1 to mediate axonal growth inhibition signaling.6,7

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human Nogo receptor. Anti-Nogo Receptor (extracellular) Antibody (#ANT-008) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NgR from human, mouse and rat samples.

For research purposes only, not for human use
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