Anti-NRG3 (extracellular) Antibody

Neuregulin-3, HRG3
  • New
Cat #: ANR-115
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)RFPGHRVPIRASPR, corresponding to amino acid residues 167 - 180 of mouse Neuregulin-3 (Accession O35181). Extracellular, N-terminus.
    Accession (Uniprot) Number O35181
    Gene ID 18183
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, rat - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ifc, ih, ip, lci
    Western blot
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1, 2. Anti-NRG3 (extracellular) Antibody (#ANR-115), (1:200).
    3, 4. Anti-NRG3 (extracellular) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell lysate (lanes 2 and 4):
    1, 2. Anti-NRG3 (extracellular) Antibody (#ANR-115), (1:200).
    3, 4. Anti-NRG3 (extracellular) Antibody, preincubated with the control peptide antigen.
    References
    1. Avramopoulos, D. (2018) Am. J. Med. Genet. B Neuropsychiatr. Genet. 177, 257.
    2. Abe, H. et al. (2017) BMC Genet. 18, 69.
    3. Tost, H. et al. (2014) J. Neurosci. 34, 1051.
    4. Hayes, L.N. et al. (2016) Mol. Neuropsychiatry 2, 79.
    Scientific background

    Neuregulins (NRGs) are single-pass membrane proteins belonging to a multi member family of epidermal growth factor (EGF)-like signaling molecules involved in cell-cell communication. This family includes four human neuregulin paralogs NRG1-NRG4 with multiple isoforms through alternative splicing1.

    Neuregulin 3 (NRG3) plays a key role in the embryonic cerebral cortex development by regulating the migration and patterning of neural progenitor cells. It specifically binds to the ErbB4 receptor tyrosine kinase in the developing mammalian forebrain2,3.

    Neuregulin 3 is almost exclusively expressed in the nervous system with highest expression in the anterior cingulate and frontal cortex3,4.

    Mutations in the neuregulin 3 gene are linked to different mental disorders accompanied with cognitive and behavioral abnormalities such as developmental delay, cognitive impairment, and psychiatric illness2,3. Single nucleotide polymorphisms are associated with delusions in patients with schizophrenia and linked to the disease itself. Knockout mice display novelty-induced hyperactivity and are deficient fear conditioning4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 20/09/2018

    Anti-NRG3 (extracellular) Antibody (#ANR-115) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Neuregulin-3 from rat, mouse, and human samples.

    For research purposes only, not for human use