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Anti-Orai1 (extracellular)-FITC Antibody

TMEM142A, Calcium release-activated calcium channel protein 1, CRACM1

Cat #: ACC-062-F
Alternative Name TMEM142A, Calcium release-activated calcium channel protein 1, CRACM1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)KFLPLKRQAGQPS, corresponding to amino acid residues 200-212 of mouse Orai1 (Accession Q8BWG9). Extracellular, 2nd loop.
Accession (Uniprot) Number Q8BWG9
Gene ID 109305
Homology Rat - identical. Will not recognize human Orai1
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity Will not recognize human Orai1
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-062), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of Orai1 by direct flow cytometry in live intact rat RBL-2H3 basophilic leukemia cells:
    Cell surface detection of Orai1 by direct flow cytometry in live intact rat RBL-2H3 basophilic leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Orai1 (extracellular)-FITC Antibody (#ACC-062-F), (5µg).
  • Cell surface detection of Orai1 by direct flow cytometry in live intact mouse TK-1 T-cell lynphoma cells:
    Cell surface detection of Orai1 by direct flow cytometry in live intact mouse TK-1
    T-cell lynphoma cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Orai1 (extracellular)-FITC Antibody (#ACC-062-F), (5µg).
References
  1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3.
  2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem99, 1503.
  3. Feske, S. et al. (2006) Nature 441, 179.
  4. Pickett, J. (2006) Nature Reviews Mol. Cell Biol. 7, 794.
  5. Luik, R.M. et al. (2006) J. Cell. Biol174, 815.
  6. Wu, M.M. et al. (2006) J. Cell. Biol174, 803.
  7. Ong, H.L. et al. (2007) J. Biol. Chem. 282, 9105.
  8. Luik, R.M and Lewis, R.S. (2007) Trends Mol. Med. 13, 103.
  9. DeHaven, W.I. et al. (2007) J. Biol. Chem. 282, 17548.
Scientific background

Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation1,2. Intracellular Ca2+ levels are controlled by either the influx of Ca2+ through the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores which gained much attention.

Recently, several key players of the store operated complex have been identified3Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.

Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Orai1 (extracellular) Antibody (#ACC-062) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize the Orai1 channel from mouse and rat samples.

Anti-Orai1 (extracellular)-FITC Antibody (#ACC-062-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

For research purposes only, not for human use
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