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Anti-OTOP1 Antibody

Proton Channel OTOP1, Otopetrin-1
Cat #: AHC-005
Alternative Name Proton Channel OTOP1, Otopetrin-1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EKSLDESKNPARKLD, corresponding to amino acid residues 374 - 388 of mouse Otopetrin-1 (Accession Q80VM9). 4th intracellular loop.
Accession (Uniprot) Number Q80VM9
Gene ID 21906
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat kidney membranes (lanes 1 and 5), mouse heart lysate (lanes 2 and 6), mouse brain lysate (lanes 3 and 7) and rat tongue lysate (lanes 4 and 8):
    Western blot analysis of rat kidney membranes (lanes 1 and 5), mouse heart lysate (lanes 2 and 6), mouse brain lysate (lanes 3 and 7) and rat tongue lysate (lanes 4 and 8):
    1-4. Anti-OTOP1 Antibody (#AHC-005), (1:200).
    5-8. Anti-OTOP1 Antibody, preincubated with OTOP1 Blocking Peptide (#BLP-HC005).
References
  1. Sautome, K. et al. (2019) Nat. Struct. Mol. Biol. 26, 518.
  2. Teng, B. et al. (2019) Curr. Biol. 29, 3647.
  3. Stewart, R.E. et al. (1998) Am. J. Physiol. 44, C227.
  4. Chen, Q. et al. (2019) Elife 8, e46710.
  5. Hughes, I. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104, 12023.
Scientific background

Otopetrin-1 (Otop-1) is a proton channel involved in sour taste recognition. It belongs to the Otopetrin proton channel family together with Otopetrins-2 and 31. Otop-1 is a dimeric structure, with each subunit forming 12 transmembrane helices divided into structurally similar N- and C-domains2.

Taste recognition is very important because it enables to sample chemicals in the environment prior to digestion and hence, plays an important role in feeding and avoiding intoxication2. The sense of sour taste is caused by acids, and the first step in sour taste transduction is an influx of H+ ions through Otop-1 channel into the type three Taste Receptor Cells (TRCs). This leads to a decrease in intracellular pH and operation of a signaling cascade1-3.

Otop-1 is specifically expressed in type three taste cells and exhibits the Zn2+ sensitivity that is a key feature observed in the proton current of sour taste cells4.

Otop-1 also has a role in regulating intracellular Ca2+ levels required for the formation of otoconia in the vertebrate inner ear. Otoconia are complex calcium carbonate biominerals required for the sensation of gravity4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 16/11/2020

Anti-OTOP1 Antibody (#AHC-005) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Otopetrin-1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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