Anti-OXGR1/GPR99 Antibody

Oxoglutarate receptor 1, 2-Oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, aKGR, GPR80, P2RY15, P2Y15
  • New
Cat #: AOR-016
Alternative Name Oxoglutarate receptor 1, 2-Oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, aKGR, GPR80, P2RY15, P2Y15
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide CKASGDLEQGKKDS, corresponding to amino acid residues 319-332 of mouse OXGR1 (Accession Q6IYF8). Intracellular, C-terminus.
        Anti-OXGR1/GPR99 Antibody
    Accession (Uniprot) Number Q6IYF8
    Gene ID 239283
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – 12/14 amino acid residues identical; human – 9/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
      • Anti-OXGR1/GPR99 Antibody
        Western blot analysis of mouse kidney (lanes 1 and 4), rat kidney (lanes 2 and 5) (1:600) and rat testis (lanes 3 and 6) (1:200) lysates:
        1-3. Anti-OXGR1/GPR99 Antibody (#AOR-016).
        4-6. Anti-OXGR1/GPR99 Antibody, preincubated with the negative control antigen.
    References
    • 1. Lazo-Fernandez, Y. et al. (2008) Am. J. Physiol. 315, F7.
    • 2. Golay, H. et al. (2019) Int. J. Mol. Sci. 20, 3889.
    • 3. Tokonami, N. et al. (2013) J. Clin. Invest. 123, 3166.
    • 4. He, W. et al. (2004) Nature 429, 188.
    • 5. Omede, A. et al. (2016) Biochem. Biophys. Res. Commun. 479, 708.
      • Oxoglutarate receptor 1 (OXGR1) is a receptor for α-ketoglutarate (αKG), a metabolite that is a citric acid cycle intermediate and a glutamine catabolism by-product1. αKG also serves as a metabolic marker and its interaction with OXGR1 has been shown to mediate the adaptive regulation of HCO3 secretion and NaCl reabsorption under acid-base stress3.

        OXGR1 belongs to the G-protein coupled receptor (GPCRs) protein family. Members of this group contain seven trans-membrane domains and respond to a large variety of ligands and thereby affect key cellular functions that are crucial for cell survival or proliferation2. OXGR1 belongs to a subset of metabolic GPCRs, along with receptors for succinate (GPR91), lactate (GPR81), fatty acids (FFAR) and many more3.

        OXGR1 is expressed in the kidney3,4 and in the heart, where it affects the contractile function, consistent with the finding that α-ketoglutarate levels are high in heart failure patients5.

        Because of its major role in maintaining cellular balance and its role in heart contractile activity, identification of molecules that can specifically activate or inhibit this receptor may be very useful in the development of novel therapeutic approach for pathological cardiac hypertrophy5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 04/11/2019

    Anti-OXGR1/GPR99 Antibody (#AOR-016) is a highly specific antibody directed against an epitope of the mouse Oxoglutarate receptor 1. The antibody can be used in western blot analysis. It has been designed to recognize OXGR1 from rat, mouse, and human samples.

    For research purposes only, not for human use