Anti-P2X2 Receptor-ATTO-594 Antibody

P2X purinoceptor 2, P2RX2
    Cat #: APR-003-AR
    Alternative Name P2X purinoceptor 2, P2RX2
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457-472 of rat P2X2 receptor (Accession P49653). Intracellular, C-terminus.
        Anti-P2X2 Receptor-ATTO-594 Antibody
    Accession (Uniprot) Number P49653
    Gene ID 114115
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Guinea pig - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical.
    RRID AB_2341044.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-003), and immunohistochemistry (labeled antibody).
    Applications: if, ih
    May also work in: ic*
      • Anti-P2X2 Receptor-ATTO-594 Antibody
        Expression of P2RX2 in rat brain
        Immunohistochemical staining of rat neocortex using Anti-P2X2 Receptor-ATTO-594 Antibody (#APR-003-AR). A. P2RX2 (red) is expressed in dendritic and axonal processes. B. Nucleus staining using DAPI as the counterstain (blue). C. Merge Images of A and B.
    References
    • 1. Prasad, M. et al. (2001) J. Physiol. 537, 667.
    • 2. Florenzano, F. et al. (2002) Neuroscience 115, 425.
    • 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego.
    • 4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
    • 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
    • 6. Lê, K.T. et al. (1998) J. Neurosci. 18, 7152.
    • 7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378.
    • 8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107.
    • 9. Kim, M. et al. (2001) EMBO J. 20, 6347.
    • 10. Chizh, B.A. and Illes.P. (2001) Pharmacol. Rev. 53, 553.
      • The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.

        The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.

        Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9

        The various mammalian P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X2 receptor subtypes has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.

        mRNA of the P2X1, P2X2, P2X3, and P2X6 receptors is found in neurons of dorsal root ganglia (DRG), trigeminal, and nodose ganglia where heteromeric P2X2/P2X3 receptors are found.10

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/11/2019

    Anti-P2X2 Receptor Antibody (#APR-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2X2 receptor from rat, mouse, and human samples.

    Anti-P2X2 Receptor-ATTO-594 Antibody (#APR-003-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-P2X2 Receptor-ATTO-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
    Citations
      • Immunohistochemical staining of mouse inner ear sections with #APR-003. Tested in P2X2R-/- mice.
        Takimoto, Y. et al. (2018) Neuroscience 386, 41.
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