Anti-P2X2 Receptor (extracellular) Antibody

P2X purinoceptor 2, P2RX2
    Cat #: APR-025
    Alternative Name P2X purinoceptor 2, P2RX2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide (C)KSDYLKHSTFDQDS, corresponding to amino acid residues 207-220 of rat P2X2 with replacement of cysteine 214 (C214) with serine (*S) (Accession P49653). Extracellular.
    • Anti-P2X2 Receptor (extracellular) Antibody
    Accession (Uniprot) Number P49653
    Gene ID 114115
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 13/14 amino acid residues identical.
    Will not recognize human P2X2.
    RRID AB_2341051.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1-2 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, lci, wb
    May also work in: ifc*, ih*, ip*
    Western blot
    • Anti-P2X2 Receptor (extracellular) Antibody
      Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and PC12 cells (lanes 3 and 6):
      1-3. Anti-P2X2 Receptor (extracellular) Antibody (#APR-025), (1:200).
      4-6. Anti-P2X2 Receptor (extracellular) Antibody, preincubated with the negative control antigen.
    Live cell imaging / Immunocytochemistry
    • Anti-P2X2 Receptor (extracellular) Antibody
      Expression of P2RX2 in rat PC12 cells
      Cell surface detection of P2RX2 in intact living rat pheochromocytoma cell line (PC12). A. Extracellular staining of cells with Anti-P2X2 Receptor (extracellular) Antibody (#APR-025), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Staining of cells with DAPI used as the counterstain. C. Merged images of A and B.
    References
    1. Prasad, M. et al. (2001) J. Physiol. 537, 667.
    2. Florenzano, F. et al. (2002) Neuroscience 115, 425.
    3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego.
    4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
    5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
    6. Lê, K.T. et al. (1998) J. Neurosci. 18, 7152.
    7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378.
    8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107.
    9. Kim, M. et al. (2001) EMBO J. 20, 6347.
    10. Chizh, B.A. and Illes.P. (2001) Pharmacol. Rev. 53, 553.
    Scientific background

    The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.

    The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.

    Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9

    The various mammalian P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X2 receptor subtypes has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.

    mRNA of the P2X1, P2X2, P2X3, and P2X6 receptors is found in neurons of dorsal root ganglia (DRG), trigeminal, and nodose ganglia where heteromeric P2X2/P2X3 receptors are found.10

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-P2X2 Receptor (extracellular) Antibody (#APR-025) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X2 Receptor from mouse, and rat samples. It is not suited to recognize human P2X2.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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