Overview
- Peptide (C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457-472 of rat P2X2 receptor (Accession P49653). Intracellular, C-terminus.
Western blot analysis of differentiated (lanes 1 and 3) and non-differentiated (lanes 2 and 4) PC12 cell lysates:1,2. Anti-P2X2 Receptor Antibody (#APR-003), (1:200).
3,4. Anti-P2X2 Receptor Antibody, preincubated with P2X2 Receptor Blocking Peptide (#BLP-PR003).
- Rat brain membranes (2 μg) (Masin, M. et al. (2006) J. Biol. Chem. 281, 4100.).
Expression of P2X2 receptor in rat neocortexImmunohistochemical staining of rat neocortex with Anti-P2X2 Receptor Antibody (#APR-003). P2X2 receptor (green) appears in axonal processes (arrows) that ascend toward the upper cortical layers (asterisk). DAPI is used as the counterstain (blue).
Expression of P2X2 receptor in rat hippocampusImmunohistochemical staining of rat hippocampus with Anti-P2X2 Receptor Antibody (#APR-003). P2X2 receptor (green) appears in the apical dendrites (arrows) that extend into the striatum radiatum (asterisk). DAPI is used as the counterstain (blue).- Mouse ilieum (1:200) (Ren, J. et al. (2003) J. Physiol. 552, 809.).
- Cultured rat myeteric cells (1:200) (Ohta, T. et al. (2005) Am. J. Physiol. 289, G935.).
- Human CD4+ and ckit+ cells (Kazakova, R.R. et al. (2011) Bull. Exp. Biol. Med. 151, 33.).
- The control antigen is not suitable for this application.
- Prasad, M. et al. (2001) J. Physiol. 537, 667.
- Florenzano, F. et al. (2002) Neuroscience 115, 425.
- Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego.
- Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
- Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
- Lê, K.T. et al. (1998) J. Neurosci. 18, 7152.
- Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378.
- Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107.
- Kim, M. et al. (2001) EMBO J. 20, 6347.
- Chizh, B.A. and Illes.P. (2001) Pharmacol. Rev. 53, 553.
The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.
The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.
Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9
The various mammalian P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X2 receptor subtypes has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.
mRNA of the P2X1, P2X2, P2X3, and P2X6 receptors is found in neurons of dorsal root ganglia (DRG), trigeminal, and nodose ganglia where heteromeric P2X2/P2X3 receptors are found.10
Anti-P2X2 Receptor Antibody (#APR-003) is a highly specific antibody directed against an intracellular epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2X2 receptor from rat, mouse, and human samples.
Expression of P2X2 receptor in rat SCN.Immunocytochemical staining of rat suprachiasmatic nuclei (SCN) cell culture. Fixed and permeabilized cells were stained using Anti-P2X2 Receptor Antibody (#APR-003). P2RX2 immunoreactivity (red) is observed in punctate structures in the SCN cell culture. Arrowheads indicate example structures that are double-labeled with P2RX2 and synapsin I (green). Dapi is used to label cell nuclei. Scale bar, 20 μm.Adapted from Bhattacharya, A. et al. (2013) with permission of The Society for Neuroscience.Applications
Citations
Powered by Bioz- Immunohistochemical staining of mouse inner ear sections. Tested in P2X2R-/- mice.
Takimoto, Y. et al. (2018) Neuroscience 386, 41.
- Mouse kidney lysates (1:500).
de Baaij, J.H.F. et al. (2016) PLoS ONE 11, e0156803. - HEK293-TSA 201 transfected cell lysates (1:5000).
Allsopp, R. and Evans, R.J. (2015) J. Biol. Chem. 290, 14556.
- Rat brain membranes (2 μg).
Masin, M. et al. (2006) J. Biol. Chem. 281, 4100.
- Mouse vestibular labyrinth sections.
Jeong, J. et al. (2020) Hearing Res. 386, 107860. - Mouse inner ear sections. Also tested in P2X2R-/- mice.
Takimoto, Y. et al. (2018) Neuroscience 386, 41. - Mouse olfactory bulb sections.
Stratford, J.M. et al. (2017) Comp. Neurol. 525, 271. - Mouse medulla sections.
Breza, J.M. and Travers, S.P. (2016) Chem. Senses 41, 515. - Mouse tongue sections (1:300).
Voigt, A. et al. (2015) J. Neurosci. 35, 9717. - Rat spinal cord sections.
Vazquez-Villoldo, N. et al. (2014) Glia 62, 171. - Mouse brainstem sections (1:500).
Ganchrow, D. et al. (2014) J. Comp. Neurol. 522, 1565. - Rat paraganglia (1:500).
Piskuric, N.A. et al. (2013) Exp. Physiol. 99, 248. - Mouse ilieum (1:200).
Ren, J. et al. (2003) J. Physiol. 552, 809.
- Human ESC NPs (1:100).
Forostyak, O. et al. (2013) Stem Cells Dev. 22, 1506. - Rat suprachiasmatic nuclei (SCN) culture.
Bhattacharya, A. et al. (2013) J. Neurosci. 33, 8035. - Cultured rat myeteric cells (1:200).
Ohta, T. et al. (2005) Am. J. Physiol. 289, G935.
- Mouse vestibular labyrinth sections.
Jeong, J. et al. (2020) Hearing Res. 386, 107860.
- Human CD4+ and ckit+ cells.
Kazakova, R.R. et al. (2011) Bull. Exp. Biol. Med. 151, 33.
- Housley, G.D. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 7494.
- Kim, M. et al. (2001) EMBO J. 20, 6347.
- Kim, M. et al. (2001) J. Biol. Chem. 276, 23262.
- Rubio, M.E., and Soto, F. (2001) J. Neurosci. 21, 641.
- Atkinson, L. et al. (2000) Neuroscience 99, 683.
- D'Ambrosi, N. et al. (2000) J. Neuropharm. 39, 1083.
- Gitterman, D.P. and Evans, R.J. (2000) Br. J. Pharmacol. 131, 1561.
- Lewis, C.J., and Evans, R.J. (2000) Br. J. Pharmacol. 131, 1659.
- Vial, C. and Evans, R.J. (2000) Br. J. Pharmacol. 131, 1489.
- Zhang, M. et al. (2000) J. Physiol. 525.1, 143.
