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Anti-P2X4 Receptor (extracellular)-FITC Antibody

P2RX4, P2X purinoceptor 4, ATP receptor
Cat #: APR-024-F
Alternative Name P2RX4, P2X purinoceptor 4, ATP receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RDLAGKEQRTLTK, corresponding to amino acid residues 301-313 of rat P2X4 receptor (Accession P51577). Extracellular.
Accession (Uniprot) Number P51577
Gene ID 29659
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-024), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection of P2RX4 in live intact mouse BV-2 microglia cells:
    Cell surface detection of P2RX4 in live intact mouse BV-2 microglia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-P2X4 Receptor (extracellular)-FITC Antibody (#APR-024-F), (2.5 µg).
  • Cell surface detection of P2RX4 in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of P2RX4 in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-P2X4 Receptor (extracellular)-FITC Antibody (#APR-024-F), (2.5 µg).
References
  1. Prasad, M. et al. (2001) J. Physiol. 537, 667.
  2. Florenzano, F. et al. (2002) Neuroscience 115, 425.
  3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego.
  4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
  5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
  6. Le, K.T. et al. (1998) J. Neurosci. 18, 7152.
  7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378.
  8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107.
  9. Kim, M. et al. (2001) EMBO J. 20, 6347.
  10. Inoue, K. et al. (2004) J. Pharmacol. Sci. 94, 112.
Scientific background

The P2X purinergic receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.

The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.

Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop.

All P2X subunits can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only appears to function as part of a heteromeric complex.4-9

The various P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4

The P2X2 receptor subunit has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.

Overexpression of P2X4 was demonstrated in microglia and in the spinal dorsal horn following peripheral nerve injury. It has been suggested that activation of P2X4 along with p38 MAPK is essential for the development of allodynia (pain from a stimulus that doesn't normally elicit pain) following nerve injury. Inhibition of P2X4 expression in spinal microglia has been suggested as a novel therapeutic approach for the treatment of allodynia.10

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-P2X4 Receptor (extracellular) Antibody (#APR-024) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell imaging, and immunocytochemistry applications. Anti-P2X4 Receptor (extracellular) Antibody recognizes an extracellular epitope and is thus ideal for cell surface detection of the receptor in living cells. It has been designed to recognize P2X4 purinergic receptor from mouse, human, and rat samples.

Anti-P2X4 Receptor (extracellular)FITC Antibody (#APR-024-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Direct flow cytometry citations
  1. Mouse monocytes and microglia.
    Srivastava, P. et al. (2020) Exp. Neurol. 329, 113308.
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