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Anti-P2X7 Receptor (extracellular)-FITC Antibody

Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Cat #: APR-008-F
Alternative Name Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
  • KO Validated
    • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.
    Accession (Uniprot) Number Q9H244
    Gene ID 64805
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, rat - identical; bovine - 14/17 amino acid residues identical.
    RRID AB_2040063.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-008), and direct flow cytometry (labeled antibody).
    Applications: fc, ic, if, lci
    Direct flow cytometry
    • Cell surface detection of P2RX7 in live intact mouse BV-2 microglia cells:
      Cell surface detection of P2RX7 in live intact mouse BV-2 microglia cells:
      ___ Cells.
      ___ Cells + rabbit IgG isotype control-FITC.
      ___ Cells + Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F), (5 µg).
    • Cell surface detection of P2RX7 in live intact human THP-1 monocytic leukemia cells:
      Cell surface detection of P2RX7 in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + rabbit IgG isotype control-FITC.
      ___ Cells + Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F), (5 µg).
    • The control antigen is not suitable for this application.
    Live cell imaging / Immunocytochemistry
    • Expression of P2RX7 in human K562 cells
      Expression of P2RX7 in human K562 cells
      Cell surface detection of P2RX7 in living K562 cells stained with Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F).
    References
    1. Ding, Y. et al. (2000) J. Auton. Nerv.Syst. 81, 289.
    2. Kim, M. et al. (2001) EMBO J. 20, 6347.
    3. Chizh, B.A. et al. (2000) Pharmacol. Rev. 53, 553.
    4. Nihei, O.K. et al. (2000) Mem.Inst.Oswaldo Cruz. 95, 415.
    Scientific background

    The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP.  To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7.  

    The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflamatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.1-3

    Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized Powder
    Image & Title:

    Anti-P2X7 Receptor (extracellular)-FITC Antibody
    Multiplex staining of P2X7 receptor and cholera toxin in MC3T3-E1 cells.    Immunocytochemical staining with labeled cholera toxin and Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F). The ganglioside GM1, the receptor for cholera toxin is concentrated in caveolae. Staining shows that P2X7 receptor colocalizes with cholera toxin.
    Adapted from Gangadharan, V.et al. (2014) with permission of the American Physiological Society.

    Last update: 08/01/2023

    Anti-P2X7 Receptor (extracellular) Antibody (#APR-008) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples.

    Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry and immunocytochemistry.

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    For research purposes only, not for human use

    Applications

    Citations

    Citations
    KO validation citations
    1. Western blot analysis of mouse neutrophil lysate using #APR-008. Tested in P2X7-/- mice.
      Karmakar, M. et al. (2016) Nat. Commun. 7, 10555.
    Direct flow cytometry citations
    1. Human PBMCs.
      Luchting, B. et al. (2016) J. Neuroinflammation 13, 1.
    More product citations
    1. Gangadharan, V. et al. (2014) Am. J. Physiol. 308, C41.
    2. Martel Gallegos, G. et al. (2010) Purinergic Signal. 6, 297.
    3. Milius, D. et al. (2008) Neurosci. Lett. 446, 45.
    4. Milius, D. et al. (2007) Toxicology 238, 60.

    Specifications

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