- Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.
- Cell surface detection of P2RX7 in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + rabbit IgG isotype control-PE.
___ Cells + Anti-P2X7 Receptor (extracellular)-PE Antibody (#APR-008-PE), (2.5 µg). - Cell surface detection of P2RX7 in live intact mouse BV-2 microglia cells:___ Cells.
___ Cells + rabbit IgG isotype control-PE.
___ Cells + Anti-P2X7 Receptor (extracellular)-PE Antibody (#APR-008-PE), (5 µg).
- Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
- Kim, M. et al. (2001) EMBO J. 20, 6347.
- Chizh, B.A. et al. (2000) Pharmacol. Rev. 53, 553.
- Nihei, O.K. et al. (2000) Mem. Inst. Oswaldo Cruz. 95, 415.
The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7.
The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflammatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis1-3.
Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization, and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx, and cell phenotype4.
Application key:
Species reactivity key:
Anti-P2X7 Receptor (extracellular) Antibody (#APR-008) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples.
Anti-P2X7 Receptor (extracellular)-PE Antibody (#APR-008-PE) is directly conjugated to Phycoerythrin (PE). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
Applications
Citations
Specifications
Scientific Background
Related Products
- Anti-P2X7 (extracellular) Antibody (#APR-008), (for western blot analysis).
- Anti-P2X7 Receptor Antibody (#APR-004). This antibody recognizes a different epitope and thus can also be used as a control for detecting P2X7.
- Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO). A fluorescent primary antibody. It can be used in multiplex staining studies in conjunction with any of our antibodies raised in rabbit.
- Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-008-AG). A fluorescent primary antibody. It can be used in colocalization studies in conjunction with any of our antibodies raised in rabbit.
- Anti-P2X7 Receptor (extracellular)-ATTO Fluor-633 Antibody (#APR-008-FR). A fluorescent primary antibody. It can be used in multiplex staining studies in conjunction with any of our antibodies raised in rabbit.
- Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F). This FITC-conjugated antibody can be used to detect P2X7 in live cell flow cytometry.
- Anti-P2X7 Receptor (extracellular) Antibody (#APR-008). This antibody recognizes an extracellular epitope and can be used to detect P2X7 in live cells.
- Anti-P2X7 Receptor (extracellular)-ATTO Fluor-633 Antibody (#APR-008-FR). This antibody is the conjugated version of #APR-008 and can be used to detect P2X7 in live cells.
- Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-008-AG). A fluorescent primary antibody. It can be used to detect P2X7 in live cells.
- Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F) is directly conjugated to fluorescein isothiocyanate (FITC). It can be used to detect P2X7 in live cells.
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