Overview
- Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.
- Rat brain membrane. Human K562 and HL-60 cell lines. Mouse WEHI-231 cells (1:200).
- Western blot analysis of rat brain membranes (lanes 1 and 5) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 6) and Mouse WEHI-231 B cell lymphoma (lanes 3 and 7) and human HL-60 promyelocytic leukemia cell line (lanes 4 and 8):1-4. Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:200).
5-8. Anti-P2X7 Receptor (extracellular) Antibody, preincubated with P2X7 Receptor (extracellular) Blocking Peptide (#BLP-PR008).
- Rat pancreas paraffin embedded section (1:100).
- Mouse P2X7 transfected in CHO cells (1:500). (Adriouch, S. et al. (2005) Cell. Immunol. 236, 72.).
- The control antigen is not suitable for this application.
- Rat basophilic leukemia (RBL) cells (1:100).
The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7.
The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflammatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.1-3
Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.4
Application key:
Species reactivity key:
Expression and upregulation of microglial P2X7 receptor in cancer.A. Immunohistochemical staining of of rat spinal dorsal horn using Anti-P2X7 Antibody (#APR-004) or Anti-P2X7 (extracellular) Antibody (#APR-008) shows that P2X7 co-localizes with CD11b, a microglia marker (two panels) and not with NeuN, a neuronal marker (lower right panel). Specificity of the antibody was tested on P2X7 knock-out mice (western blot, in upper panel). B. Western blot analysis of rat dorsal horn lysates shows that P2X7 expression increases on post-tumor day 14 (PTD 14).Adapted from Yang, Y. et al. (2015) with permission of the Society for Neuroscience.