Anti-P2Y1 Receptor (extracellular)-ATTO-488 Antibody

P2Y purinoceptor 1, P2RY1
    Cat #: APR-021-AG
    Alternative Name P2Y purinoceptor 1, P2RY1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1 (Accession P47900). 2nd extracellular loop.
    • Anti-P2Y1 Receptor (extracellular)-ATTO-488 Antibody
    Accession (Uniprot) Number P47900
    Gene ID 5028
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - identical; mouse - 12/14 amino acid residues identical.
    RRID AB_10918468.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-021), and Direct Flow Cytometry (labeled antibody).
    Applications: fc, ic, if, ih, lci
    • Anti-P2Y1 Receptor (extracellular)-ATTO-488 Antibody
      Expression of P2RY1 in mouse brain
      Immunohistochemical staining of mouse hippocampus using Anti-P2Y1 Receptor (extracellular)-ATTO-488 Antibody (#APR-021-AG), (1:80). A. P2RY1 staining (green) is detected in pyramidal cells (horizontal arrow) and in apical dendrites (vertical arrow). B. Nuclei staining using DAPI as the counterstain. C. Merged image of panels A and B.
    Direct flow cytometry
    • Anti-P2Y1 Receptor (extracellular)-ATTO-488 Antibody
      Cell surface detection of P2RY1 in intact living Jurkat cells:
      ___ Unstained cells.
      ___ Cells + Anti-P2Y1 Receptor (extracellular)-ATTO-488 antibody, (#APR-021-AG), (5 µg/5x105 cells.).
    Live cell imaging / Immunocytochemistry
    • Anti-P2Y1 Receptor (extracellular)-ATTO-488 Antibody
      Expression of P2RY1 in rat PC12 cells
      Cell surface detection of P2RY1 in intact living rat pheochromocytoma (PC12) cells. Extracellular staining of cells using Anti-P2Y1 receptor (extracellular)-ATTO-488 Antibody (#APR-021-AG), (1:50), (green).
    1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
    2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
    3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
    4. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
    5. Jin, J. et al. (1998) J. Biol. Chem. 273, 2030.
    Scientific background

    The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1

    The mammalian P2Y family currently includes eight functional receptors; P2Y1P2Y2P2Y4P2Y6P2Y11P2Y12P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4

    P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021) is a highly specific antibody directed against an extracellular epitope of the human P2RY1. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry and live cell imaging applications. It has been designed to recognize P2RY1 from mouse, rat and human samples.

    Anti-P2Y1 Receptor (extracellular)-ATTO-488 Antibody (#APR-021-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-P2Y1 Receptor (extracellular)-ATTO-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use



    Scientific Background


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