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Anti-P2Y10 (extracellular) Antibody

P2Y Receptor Family Member 10, Putative P2Y Purinoceptor 10, P2Y-Like Receptor, LYPSR2, P2RY10

Cat #: APR-110
Alternative Name P2Y Receptor Family Member 10, Putative P2Y Purinoceptor 10, P2Y-Like Receptor, LYPSR2, P2RY10
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)NSTDS(S)FADLGYKQMD, corresponding to amino acid residues 165 -180 of mouse P2Y10 (Accession Q8BFU7). Extracellular, 2nd loop.
Accession (Uniprot) Number Q8BFU7
Gene ID 78826
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – 14 out of 16 amino acid residues identical. Not recommended for human samples.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
P2Y10 (extracellular) Blocking Peptide (BLP-PR110)
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1-2. Anti-P2Y10 (extracellular) Antibody (#APR-110), (1:400).
    3-4. Anti-P2Y10 (extracellular) Antibody, preincubated with P2Y10 (extracellular) Blocking Peptide (BLP-PR110).
  • Western blot analysis of mouse spleen membranes (lanes 1 and 3) and rat small intestine lysate (lanes 2 and 4):
    Western blot analysis of mouse spleen membranes (lanes 1 and 3) and rat small intestine lysate (lanes 2 and 4):
    1-2. Anti-P2Y10 (extracellular) Antibody (#APR-110), (1:200).
    3-4. Anti-P2Y10 (extracellular) Antibody, preincubated with P2Y10 (extracellular) Blocking Peptide (BLP-PR110).
  • Western blot analysis of mouse WEHI-231 B-cell lymphoma cell line lysate (lanes 1 and 4), mouse BV-2 microglia cell line lysate (lanes 2 and 5) and mouse P815 mastocytoma cell line lysate (lanes 3 and 6):
    Western blot analysis of mouse WEHI-231 B-cell lymphoma cell line lysate (lanes 1 and 4), mouse BV-2 microglia cell line lysate (lanes 2 and 5) and mouse P815 mastocytoma cell line lysate (lanes 3 and 6):
    1-3. Anti-P2Y10 (extracellular) Antibody (#APR-110), (1:200).
    4-6. Anti-P2Y10 (extracellular) Antibody, preincubated with P2Y10 (extracellular) Blocking Peptide (BLP-PR110).
Immunohistochemistry
  • Expression of P2Y10 in mouse striatum.
    Expression of P2Y10 in mouse striatum.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-P2Y10 (extracellular) Antibody (#APR-110), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. P2Y10 immunoreactivity (green) appears in striatal cells (arrows). B. Pre-incubation of the antibody with P2Y10 (extracellular) Blocking Peptide (BLP-PR110), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of P2Y10 by indirect flow cytometry in live intact mouse BV-2 microglia cells:
    Cell surface detection of P2Y10 by indirect flow cytometry in live intact mouse BV-2 microglia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-P2Y10 (extracellular) Antibody (#APR-110), (5μg) + goat-anti-rabbit-FITC.
References
  1. Rao, S. et al. (1999) J. Biol. Chem. 274, 34245.
  2. Murakami, M. et al. (2008) Biochem. Biophys. Res. Commun. 371, 707.
  3. Inoue, A. et al. (2012) Nat. Methods. 9, 1021.
  4. Omi, J. et al. (2021) Cell. Biochem. Biophys. 79, 497.
  5. Hwang, S.M. et al. (2018) Clin. Exp. Allergy. 48, 990.
  6. Kita, M. et al. (2019) Sci. Rep.  9, 3135.
  7. Gurusami, M. et al. (2021) Nat. Comm. 12, 6798.
Scientific background

P2Y10 belongs to the family of G-protein coupled receptors (GPCRs) and was first described as a lymphoid-restricted receptor. Based on sequence homology, it was thought to be a member of the purinergic P2Y GPCR family and a nucleotide receptor 1. Later studies suggested that it was a receptor for lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P)2, although the findings were not confirmed by others.

More recent studies using a TGFα shedding assay, showed P2Y10 as a receptor for lysophosphatidylserine (LysoPS), a bioactive lipid present in the central nervous system (CNS) and immune system 3,4. Nevertheless, P2Y10 is still classified by the International Union of Basic and Clinical Pharmacology, as an orphan class A GPCR receptor.

P2Y10 putative ligand, LysoPS, has been found to activate at least two other class A orphan GPCRs named GPR34 and GPR174, that have also been named as LYPSR1 and LYPSR3 (respectively), with P2Y10 also named as LYPSR24.

The biological function of P2Y10 has not been completely elucidated, but different studies point to a role in eosinophil degranulation5, involvement in neuroinflammation6 and regulation of T-cell migration7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 13/06/2022

Anti-P2Y10 (extracellular) Antibody (#APR-110) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize P2Y10 from mouse and rat samples. The antibody is unlikely to recognize human P2Y10.

For research purposes only, not for human use
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