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Anti-P2Y13 Receptor (extracellular) Antibody

Purinergic Receptor P2Y13, P2RY13, GPCR1, GPR86, GPR94

Cat #: APR-103
Alternative Name Purinergic Receptor P2Y13, P2RY13, GPCR1, GPR86, GPR94
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)NKTD(S)RLENQLFIAKE, corresponding to amino acid residues 266 - 281 of mouse P2Y13 (Accession Q9D8I2). Extracellular, 3rd loop.
Accession (Uniprot) Number Q9D8I2
Gene ID 74191
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry
Homology Mouse – 15 out of 16 amino acid residues identical. Human, rat – 14 out of 16 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis
Applications: ifc, lci, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat dorsal root ganglia lysate (lanes 3 and 7) and rat spleen lysate (lanes 4 and 8):
    Western blot analysis of rat brain lysate (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat dorsal root ganglia lysate (lanes 3 and 7) and rat spleen lysate (lanes 4 and 8):
    1-4. Anti-P2Y13 Receptor (extracellular) Antibody (#APR-103), (1:200).
    5-8. Anti-P2Y13 Receptor (extracellular) Antibody, preincubated with P2Y13 Receptor (extracellular) Blocking Peptide (BLP-PR103).
  • Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 5), mouse BV-2 microglia cell line lysate (lanes 2 and 6), mouse J774 monocyte cell line lysate (lanes 3 and 7) and human HMC3 microglia cell line lysate (lanes 4 and 8):
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 5), mouse BV-2 microglia cell line lysate (lanes 2 and 6), mouse J774 monocyte cell line lysate (lanes 3 and 7) and human HMC3 microglia cell line lysate (lanes 4 and 8):
    1-4. Anti-P2Y13 Receptor (extracellular) Antibody (#APR-103), (1:200).
    5-8. Anti-P2Y13 Receptor (extracellular) Antibody, preincubated with P2Y13 Receptor (extracellular) Blocking Peptide (BLP-PR103).
Indirect flow cytometry
  • Cell surface detection of P2Y13 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of P2Y13 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-P2Y13 Receptor (extracellular) Antibody (#APR-103), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Communi, D. et al. (2001) J. Biol. Chem276, 41479.
  2. Zhang, F.L. et al. (2002) J. Pharmacol. Exp. Ther301, 705.
  3. Wang, L. et al. (2005) Circ. Res96, 189.
  4. Wirkner, K. et al. (2004) Br. J. Pharmacol141, 141.
Scientific background

The P2Y family belongs to the G-protein coupled receptors superfamily. They are activated by extracellular nucleotides and modulate variety of physiological functions.

A new member of this family was recently identified, the P2Y13 receptor.1,2  The P2Y13 receptor has significant similarity to the P2Y12 receptor (about 45% sequence identity) and together they form a distinct group structurally differ from other members of this family. P2Y13 receptor, like the P2Y12 receptor, is a high affinity receptor for the ADP nucleotide, coupled to the Gi class of G-proteins.1,2 The P2Y13 receptor is highly expressed at brain and spleen tissues as well as in the immune system. Lower expression was demonstrated in the testis, lung liver and other peripheral organs.1,2 

Recently it has been shown that ADP activates a negative feedback pathway for ATP release from human red blood cells via the P2Y13 receptor.3

P2Y13 receptor was also implicated in inhibition of N-type Ca2+ channels in neurons, exerting pre and/or post-synaptic modulatory action.4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-P2Y13 Receptor (extracellular) Antibody (#APR-103) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize P2Y13 from mouse, rat and human samples.

For research purposes only, not for human use
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