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Anti-P2Y13 Receptor (extracellular)-FITC Antibody

Purinergic Receptor P2Y13, P2RY13, GPCR1, GPR86, GPR94, SP174

Cat #: APR-103-F
Alternative Name Purinergic Receptor P2Y13, P2RY13, GPCR1, GPR86, GPR94, SP174
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)NKTD(S)RLENQLFIAKE, corresponding to amino acid residues 266 - 281 of mouse P2Y13 (Accession Q9D8I2). Extracellular, 3rd loop. 
Accession (Uniprot) Number Q9D8I2
Gene ID 74191
Homology Mouse – 15 out of 16 amino acid residues identical. Human, rat – 14 out of 16 amino acid residues identical. 
Purity Affinity purified on immobilized antigen.
Form Lyophilized powderReconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. 
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC) 
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-103), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of P2Y13 by direct flow cytometry in live intact mouse BV-2 microglia cells:
    Cell surface detection of P2Y13 by direct flow cytometry in live intact mouse BV-2 microglia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-P2Y13 Receptor (extracellular)-FITC Antibody(#APR-103-F), (5µg) .
  • Cell surface detection of P2Y13 by direct flow cytometry in live intact mouse J774 macrophage cells: 
    Cell surface detection of P2Y13 by direct flow cytometry in live intact mouse J774 macrophage cells: 
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-P2Y13 Receptor (extracellular)-FITC Antibody(#APR-103-F), (2.5µg).
References
  1. Communi, D.et al. (2001) J. Biol. Chem.276, 41479.
  2. Zhang, F.L.et al. (2002)J. Pharmacol. Exp. Ther.301, 705.
  3. Wang, L.et al. (2005) Circ. Res.96, 189
  4. Wirkner, K.et al. (2004) Br. J. Pharmacol.141, 141.
Scientific background

The P2Y family belongs to the G-protein coupled receptors superfamily. They are activated by extracellular nucleotides and modulate variety of physiological functions. 

A new member of this family was recently identified, the P2Y13 receptor.1,2 The P2Y13 receptor has significant similarity to the P2Y12 receptor (about 45% sequence identity) and together they form a distinct group structurally differ from other members of this family. P2Y13 receptor, like the P2Y12 receptor, is a high affinity receptor for the ADP nucleotide, coupled to the Gi class of G-proteins.1,2 The P2Y13 receptor is highly expressed at brain and spleen tissues as well as in the immune system. Lower expression was demonstrated in the testis, lung, liver and other peripheral organs.1,2 

Recently it has been shown that ADP activates a negative feedback pathway for ATP release from human red blood cells via the P2Y13 receptor.3 

P2Y13 receptor was also implicated in inhibition of N-type Ca2+ channels in neurons, exerting pre and/or post-synaptic modulatory action.4 

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 22/08/2021

Anti-P2Y13 Receptor (extracellular) Antibody (#APR-103) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize P2Y13 from mouse, rat and human samples.

Anti-P2Y13 Receptor (extracellular)-FITC Antibody (#APR-103-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

For research purposes only, not for human use
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