Overview
- Peptide (C)KPAY GTTGL PRAKR KSVR, corresponding to residues 227-244 of rat P2RY2 (Accession P41232). 3rd intracellular loop (i3) between TM5 and TM6 domains.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1, 2. Anti-P2Y2 Receptor Antibody (#APR-010), (1:200).
3, 4. Anti-P2Y2 Receptor Antibody, preincubated with P2Y2 Receptor Blocking Peptide (#BLP-PR010). - Rat brain membranes, rat lung membranes (1:200).
Mouse mIMCD-K2 cell lysate (4 μg/ml) (Rajagopal, M. et al. (2011) Am. J. Physiol. 301, F544.).
- Rat DRGs (Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.).
- Rat dorsal root ganglia (1:300) (Mo, G. et al. (2013) Mol. Pharmacol. 83, 640.).
- Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
- Rat PC12 cells (1:100) (Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.).
- Rat osteoclasts (8 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
- The control antigen is not suitable for this application.
The P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation.1
The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14 .2-4
The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway.5 It has become a leading candidate for the treatment of cystic fibrosis. Activation of P2Y2 receptor increases Cl- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis.6
Application key:
Species reactivity key:
Expression of P2Y2 receptor in rat cortical neurons.Immunocytochemical staining of rat cortical neurons using Anti-P2Y2 Receptor Antibody (#APR-010). P2Y2 receptor immuno-colocalizes with PSD-95, the synaptic marker.Adapted from Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50. with permission of the American Society for Pharmacology & Experimental Therapeutics.