Anti-P2Y6 Receptor (extracellular) Antibody

P2Y purinoceptor 6, P2RY6
  • New
Cat #: APR-106
Alternative Name P2Y purinoceptor 6, P2RY6
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RNRTV(S)YDLSPPILS, corresponding to amino acid residues 172 - 186 of mouse P2RY6 (Accession Q9ERK9). 2nd extracellular loop.
    Accession (Uniprot) Number Q9ERK9
    Gene ID 233571
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic, ih, ip, lci
      • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) heart membranes:
        1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:400).
        3,4. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
        Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
        1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:200).
        3,4. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
        Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate:
        1. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:200).
        2. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
      • Indirect flow cytometry of live intact mouse J774 macrophage cells:
        ___ Cells.
        ___ Cells + goat-anti-rabbit-FITC.
        ___ Cells + Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (2.5 µg) + goat-anti-rabbit-FITC.
      • The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily1,3.

        P2Y6 agonists induce an inositol phosphate/Ca2+ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca2+ and M-type K+ channels1,2.

        P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 04/04/2019

    Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize P2RY6 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
    Related Products
        • Anti-P2Y6 Receptor Antibody (#APR-011). This antibody recognizes a different epitope and thus can also be used as a control for detecting P2Y6.