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Anti-P2Y6 Receptor (extracellular)-FITC Antibody

P2Y purinoceptor 6, P2RY6
Cat #: APR-106-F
Alternative Name P2Y purinoceptor 6, P2RY6
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RNRTV(S)YDLSPPILS, corresponding to amino acid residues 172-186 of mouse P2RY6 (Accession Q9ERK9). 2nd extracellular loop.
Accession (Uniprot) Number Q9ERK9
Gene ID 233571
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-106), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection of P2Y6 in live intact mouse BV-2 microglia cells:
    Cell surface detection of P2Y6 in live intact mouse BV-2 microglia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-P2Y6 Receptor (extracellular)-FITC Antibody (#APR-106-F), (2.5 µg).
  • Cell surface detection of P2Y6 in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of P2Y6 in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-P2Y6 Receptor (extracellular)-FITC Antibody (#APR-106-F), (2.5 µg).
References
  1. Communi, D. et al. (2000) Cell Signal. 12, 351.
  2. Filippov, A.K. et al. (1999) Br. J. Pharmacol. 126, 1009.
  3. Chang, K. et al. (1995) J. Biol. Chem. 270, 26152.
  4. Malmsjo, M. et al. (2003) B.M.C. Pharmacol. 3, 4.
Scientific background

The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily1,3.

P2Y6 agonists induce an inositol phosphate/Ca2+ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca2+ and M-type K+ channels1,2.

P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry applications. It has been designed to recognize P2RY6 from human, rat, and mouse samples.

Anti-P2Y6 Receptor (extracellular)-FITC Antibody (#APR-106-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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