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Anti-P2Y6 Receptor Antibody

P2Y purinoceptor 6, P2RY6

Cat #: APR-011
Alternative Name P2Y purinoceptor 6, P2RY6
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CQPHDLLQKLTAKWQRQRV, corresponding to amino acid residues 311-328 of rat P2Y6 receptor (Accession Q63371). Intracellular, C-terminus.
    Accession (Uniprot) Number Q63371
    Gene ID 117264
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical.
    RRID AB_2040082.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, ip, wb
    Western blot
    • Western blot analysis of rat heart membranes:
      Western blot analysis of rat heart membranes:
      1. Anti-P2Y6 Receptor Antibody (#APR-011), (1:200).
      2. Anti-P2Y6 Receptor Antibody, preincubated with P2Y6 Receptor Blocking Peptide (#BLP-PR011).
    Immunoprecipitation
    • Mouse theca intertitial cells (3 μl) (Vazquez Cuevas, F.G. et al. (2010) Reprod. Biol. Endocrinol. 8, 88.).
    Immunohistochemistry
    • Mouse paraffin heart section.
    • Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
    • Expression of P2Y6 in rat cortex. 
      Expression of P2Y6 in rat cortex. 
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y6 Receptor Antibody (#APR-011), (1:600), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. P2Y6 immunoreactivity (red) appears in cortical neurons (horizontal arrows) and astrocytes (vertical arrows). B. Pre-incubation of the antibody with P2Y6 Receptor Blocking Peptide (BLP-PR011), suppressed staining. Cell nuclei are stained with DAPI (blue).
    Immunocytochemistry
    • Human P2Y6 SHSY-5Y transfected cells (1:500) (Apolloni, S. et al. (2010) Neurochem. Int. 56, 670.).
    Indirect flow cytometry
    • Rat osteoclasts (6 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
    • The control antigen is not suitable for this application.
    References
    1. Communi, D. et al. (2000) Cell Signal. 12, 351.
    2. Filippov, A.K. et al. (1999) Br. J. Pharmacol. 126, 1009.
    3. Chang, K. et al. (1995) J. Biol. Chem. 270, 26152.
    4. Malmsjo, M. et al. (2003) B.M.C. Pharmacol. 3, 4.
    Scientific background

    The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily.1,3

    P2Y6 agonists induce an inositol phosphate/Ca2+ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca2+ and M-type K+ channels.1,2

    P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries.4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 02/08/2022

    Anti-P2Y6 Receptor Antibody (#APR-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY6 from rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Citations

    Citations
    KO validation citations
    1. Western blot and indirect flow cytometry. Tested in NK cells from P2Y6 -/- knockout mice.
      Li, Z. et.al. (2021) J. Immunol. 207, 1555.
    Western blot citations
    1. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    Immunoprecipitation citations
    1. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Mouse theca intertitial cells (3 μl).
      Vazquez Cuevas, F.G. et al. (2010) Reprod. Biol. Endocrinol. 8, 88.
    Immunohistochemistry citations
    1. Mouse brain sections (1:100).
      Ribeiro Xavier, A.L. et al. (2015) J. Neurosci. 35, 11848.
    2. Human eye tissue.
      Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.
    Immunocytochemistry citations
    1. Human P2Y6 SHSY-5Y transfected cells (1:500).
      Apolloni, S. et al. (2010) Neurochem. Int. 56, 670.
    Indirect flow cytometry citations
    1. Mouse bone marrow cells.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Rat osteoclasts (6 μg/ml).
      Alvarenga, E.C. et al. (2010) Bone 46, 355.
    More product citations
    1. Balasubramanian, R. et al. (2013) Biochem. Pharmacol. 85, 991.
    2. Traves, P.G. et al. (2013) J. Immunol. 190, 4226.
    3. Yu, W. et al. (2013) FASEB J. 27, 1895.
    4. Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.

    Specifications

    Scientific Background

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