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Anti-pan ASIC (extracellular) Antibody

Acid-sensing ion channel, Acid-sensing (proton-gated) ion channel, ACCN, ACCN2, ACCN3, ACCN4

Cat #: ASC-031
Alternative Name Acid-sensing ion channel, Acid-sensing (proton-gated) ion channel, ACCN, ACCN2, ACCN3, ACCN4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)TRYGKELSMVKIPSK, corresponding to amino acid residues 368-382 of rat ASIC1 (Accession P55926), amino acid residues 367–381 of rat ASIC2 (Accession Q62962), amino acid residues 375–388 of rat ASIC3 (Accession O35240) and amino acid residues 378–390 of rat ASIC4 (Accession Q9JHS6). Extracellular loop.
Accession (Uniprot) Number P55926
Gene ID 79123
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology ASIC1: Mouse, rat, human – identical.
ASIC2: Mouse, rat, human – 14/15 amino acid residues identical.
ASIC3: Mouse, rat, human – 12/15 amino acid residues identical.
ASIC4: Mouse, rat – 12/15 amino acid residues identical; human – 11/15 amino acid residues identical.
RRID AB_2756791.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, lci, wb
May also work in: ip*
Western blot
  • Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1-2. Anti-pan ASIC (extracellular) Antibody (#ASC-031), (1:500).
    3-4. Anti-pan ASIC (extracellular) Antibody, preincubated with Pan ASIC (extracellular) Blocking Peptide (#BLP-SC031).
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 4):
    1-2. Anti-pan ASIC (extracellular) Antibody (#ASC-031), (1:500).
    3-4. Anti-pan ASIC (extracellular) Antibody, preincubated with Pan ASIC (extracellular) Blocking Peptide (#BLP-SC031).
Immunohistochemistry
  • Expression of ASIC channels in rat locus coeruleus
    Expression of ASIC channels in rat locus coeruleus
    Immunohistochemical staining of perfusion-fixed rat brain frozen sections using Anti-pan ASIC (extracellular) Antibody (#ASC-031), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. ASIC channel staining (green) appears in neuronal soma (arrows). Nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of  ASIC by Indirect flow cytometry in live intact mouse macrophage J774 cells : 
    Cell surface detection of  ASIC by Indirect flow cytometry in live intact mouse macrophage J774 cells : 
    ___ Cells.
    ___ Cells + goat-anti-rabbit-APC.
    ___ Cells + Anti-pan ASIC (extracellular) Antibody (#ASC-031), (5μg) + goat-anti-rabbit-APC.
Live cell imaging / Immunocytochemistry
  • Expression of ASIC in rat PC12 cells
    Expression of ASIC in rat PC12 cells
    Cell surface detection of ASIC in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-pan ASIC (extracellular) Antibody (#ASC-031), (1:25), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge of A and B.
References
  1. Poirot, O. et al. (2004) J. Biol. Chem. 279, 38448.
  2. Krishtal, O. (2003) Trends. Neurosci. 26, 477.
  3. Brockway, L.M. et al. (2002) Am. J. Physiol. 283, C126.
  4. Ishibashi, K. et al. (1998) Biochem. Biophys. Res. Commun. 245, 589.
  5. Voilley, N. et al. (2001) J. Neurosci. 21, 8026.
  6. Wemmie, J.A. et al. (2002) Neuron 34, 463.
  7. Price, M.P. et al. (2000) Nature 407, 1007.
Scientific background

ASIC channels belong to the ENaC/Degenerin superfamily and are proton-gated ion channels which are not voltage-dependent. Six different genes encode the different ASIC subunits and splice variants: ASIC1a, ASIC1b, ASIC2a, ASIC2b, ASIC3 and ASIC4.

ASIC channels sense protons and interact with proteins (namely proteases) and other ions such as Zn2+ and Ca2+ through their extracellular domains. The extracellular loop of ASIC1a undergoes cleavage by extracellular trypsin (a serine protease) which is responsible for shifting the pH dependence of the channel activation and inactivation to more acidic pH1.

These channels are expressed in the brain and in the nervous system2 but have also been found in the retina, testes, and other tissues3,4 where they are involved in many physiological processes including nociception5, learning and memory6, mechanosensation7 among others.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 08/01/2023

Alomone Labs is pleased to offer an antibody directed against an epitope of rat ASIC1. The antibody recognizes ASIC1 through ASIC4. Anti-pan ASIC (extracellular) Antibody (#ASC-031) can be used in western blot, immunohistochemistry and immunocytochemistry in living cells. It has been designed to recognize all ASIC channels from rat, human and mouse samples.

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For research purposes only, not for human use

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