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Guinea pig Anti-Pan NaV Antibody

Pan sodium channel, SP19
Cat #: AGP-007
Alternative Name Pan sodium channel, SP19
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: h, m, r
Immunogen
  • Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
Accession (Uniprot) Number P04774
Gene ID 81574
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels.
RRID AB_2340959.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    1,2. Guinea pig Anti-Pan NaV Antibody (#AGP-007), (1:800).
    3,4. Guinea pig Anti-Pan NaV Antibody, preincubated with Pan Nav Blocking Peptide (#BLP-SC003).
References
  1. Wu, L. et al. (2002) NeuroReport 13, 2547.
  2. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
  3. Fjell, J. et al. (2000) NeuroReport 11, 199.
  4. Baker, M.D. et al. (2001) Trends. Pharmacol. Sci. 22, 27.
  5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
  6. Isom, L.L. (2001) Neuroscientist 7, 42.
  7. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
Scientific background

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5  The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer an antibody directed against an epitope of the rat NaV1.1 channel. The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels. Guinea pig Anti-Pan NaV Antibody (#AGP-007), raised in guinea pigs, can be used in western blot analysis, and has been designed to recognize NaV channels from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Pan NaV Antibody (#ASC-003) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use
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