- Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
- Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Guinea pig Anti-Pan NaV Antibody (#AGP-007), (1:800).
3,4. Guinea pig Anti-Pan NaV Antibody, preincubated with Pan Nav Blocking Peptide (#BLP-SC003).
- Wu, L. et al. (2002) NeuroReport 13, 2547.
- Fang, X. et al. (2002) J. Neurosci. 22, 7425.
- Fjell, J. et al. (2000) NeuroReport 11, 199.
- Baker, M.D. et al. (2001) Trends. Pharmacol. Sci. 22, 27.
- Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
- Isom, L.L. (2001) Neuroscientist 7, 42.
- Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3
Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7
Species reactivity key:
Alomone Labs is pleased to offer an antibody directed against an epitope of the rat NaV1.1 channel. The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels. Guinea pig Anti-Pan NaV Antibody (#AGP-007), raised in guinea pigs, can be used in western blot analysis, and has been designed to recognize NaV channels from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Pan NaV Antibody (#ASC-003) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
- Lee, C. et al. (2015) Biochem. Biophys. Res. Commun. 463, 1203.