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Anti-Pannexin 1 Antibody

PANX1, PX1, MRS1, UNQ2529
Cat #: ACC-234
Alternative Name PANX1, PX1, MRS1, UNQ2529
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KEPTEPKFKGLRLE, corresponding to amino acid residues 18- 31 of human PANX1 (Accession Q96RD7). Intracellular, N-terminus.
    Accession (Uniprot) Number Q96RD7
    Gene ID 24145
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - identical.
    RRID AB_2340917.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Western blot analysis of mouse (lanes 1 and 5) and rat (lanes 2 and 6) heart lysates and mouse (lanes 3 and 7) and rat (lanes 4 and 8) brain membranes:
      Western blot analysis of mouse (lanes 1 and 5) and rat (lanes 2 and 6) heart lysates and mouse (lanes 3 and 7) and rat (lanes 4 and 8) brain membranes:
      1-4. Anti-Pannexin 1 Antibody (#ACC-234), (1:400).
      5-8. Anti-Pannexin 1 Antibody, preincubated with Pannexin 1 Blocking Peptide (#BLP-CC234).
    Immunohistochemistry
    • Expression of Pannexin 1 in rat cortex
      Expression of Pannexin 1 in rat cortex
      Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Pannexin 1 Antibody (#ACC-234), (1:300). PANX1 staining (green) is apparent in cells with neuronal outline (vertical arrows) and apical dendrites (horizontal arrows) in the parietal cingulated cortex region. Cell nuclei are stained with DAPI (blue).
    References
    1. Chew, S.S. et al. (2010) Exp. Neurol. 225, 250.
    2. Nakagawa, S. et al. (2010) Curr. Opin. Struct. Biol. 20, 423.
    3. Trexler, E.B. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 5836.
    4. Hu, X. et al. (2006) Biophys. J. 90, 140.
    5. Shestopalov, V.I. and Panchin, Y. (2008) Cell. Mol. Life Sci. 65, 376.
    6. Unger, V.M. et al. (1999) Science 283, 1176.
    7. Falk, M.M. (2000) Eur. J. Cell. Biol. 79, 564.
    8. Panchin, Y.V. (2005) J. Exp. Biol. 208, 1415.
    9. Litvin, O. et al. (2006) J. Cell. Mol. Med. 10, 613.
    10. Bruzzone, R. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 13664.
    11. Bravo, D. et al. (2015) Pharmacol. Res. 101, 86.
    Scientific background

    Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells1. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2-4.

    Proteins involved in gap junction formation are composed of four transmembrane domains, two extracellular loops and one intracellular loop and intracellular N- and C-termini5,6. Several consensus cysteine residues are in the extracellular loop and are essential and necessary for intercellular docking of gap junction hemichannels in the opposing cell membrane5-7.

    Pannexins (Pannexin 1, Pannexin 2 and Pannexin 3) belong to the superfamily of gap junction proteins. Pannexin 1 (PANX1) is ubiquitously expressed, Pannexin 2 (PANX2) is specifically expressed in the human brain and widespread in rodents and Pannexin 3 (PANX3) is also detected in the brain8.

    The gating properties of Pannexins were studied in Xenopus oocytes and results demonstrate that only PANX1 is able to form homomeric hemichannels, and is also able to form heteromeric hemichannels with PANX2 but not with PANX39,10. Not surprising, PANX1 gating properties depend whether it forms homomeric or heteromeric hemichannels.

    A number of different stimuli are known to open these channels and include mechanical stress, extracellular ATP, increases in intracellular Ca2+ and inflammation9.

    Possible roles for Pannexins include paracrine signaling in vascular endothelial cells and taste cell signaling9. Pannexin-1 was shown to interact with NMDA receptors in epilepsy, ischemia, pain and other pathologies10.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Pannexin 1 AntibodyExpression of Pannexin 1 in mouse liver and carotid arteries.A. Western blot analysis of mouse liver lysate using Anti-Pannexin 1 Antibody (#ACC-234). PANX1 is not detected in knockout mice. B. Immunohistochemical staining of mouse carotid artery sections demonstrates PANX1 expression (green) in endothelial cells. No expression is observed in knockout animals (middle panel) nor under conditions where Anti-Pannexin 1 Antibody was omitted (right panel).Adapted from Molica, F. et al. (2017) Sci. Rep. 7, 13706. with permission of Nature Publishing Group.

    Last update: 23/08/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Pannexin 1. Anti-Pannexin 1 Antibody (#ACC-234) can be used in western blot and immunohistochemistry applications. It was designed to recognize PANX1 from rat, mouse and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Western blot analysis of mouse liver and endothelium-denudated thoracic abdominal aorta lysate. Immunohistochemical staining of mouse carotid artery sections. Tested in PANX1-/- mice.
      Molica, F. et al. (2017) Sci. Rep. 7, 13706.
    Western blot citations
    1. Mouse liver and endothelium-denudated thoracic abdominal aorta lysate. Also tested in PANX1-/- mice.
      Molica, F. et al. (2017) Sci. Rep. 7, 13706.
    2. Rat bladder lysate.
      Beckel, J.M. et al. (2015) J. Physiol. 593, 1857.
    Immunohistochemistry citations
    1. Rat brainstem sections (1:300).
      Corsini, S. et al. (2017) Cell Death Dis. 8, e2881.
    2. Mouse carotid artery sections. Also tested in PANX1-/- mice.
      Molica, F. et al. (2017) Sci. Rep. 7, 13706.
    3. Rat bladder sections.
      Beckel, J.M. et al. (2015) J. Physiol. 593, 1857.
    4. Mouse ganglia sections.
      Feldman-Goriachnik, R. et al. (2015) Glia 63, 2121.
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