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Anti-PTH1R (extracellular) Antibody

Parathyroid hormone 1 receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR, PTHR1
Cat #: APR-051
Alternative Name Parathyroid hormone 1 receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR, PTHR1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DAVLYSGFTLDEAER, corresponding to amino acid residues 241-255 of rat PTH1R (Accession P25961). 1st extracellular loop.
Accession (Uniprot) Number P25961
Gene ID 56813
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 14/15 amino acid residues identical.
RRID AB_2341053.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of HepG2 (lanes 1 and 4), mouse liver (lanes 2 and 5) and rat liver (lanes 3 and 6) lysates:
    Western blot analysis of HepG2 (lanes 1 and 4), mouse liver (lanes 2 and 5) and rat liver (lanes 3 and 6) lysates:
    1-3. Anti-PTH1R (extracellular) Antibody (#APR-051), (1:200).
    4-6. Anti-PTH1R (extracellular) Antibody, preincubated with PTH1R (extracellular) Blocking Peptide (#BLP-PR051).
Immunohistochemistry
  • Expression of Parathyroid hormone receptor 1 in rat brain
    Expression of Parathyroid hormone receptor 1 in rat brain
    Immunohistochemical staining of rat dorsal Raphe nucleus using Anti-PTH1R (extracellular) Antibody (#APR-051). A. PTH1R (red) staining is detected in Raphe neurons (arrows). B. Nucleus staining using DAPI as the counterstain (blue). C. Merged images of A and B.
  • Multiplex staining of Parathyroid hormone receptor 1 and Orexin receptor 1 in rat ventromedial hypothalamus
    Multiplex staining of Parathyroid hormone receptor 1 and Orexin receptor 1 in rat ventromedial hypothalamus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody (#AOR-001-AG), (1:60) and Anti-PTH1R (extracellular) Antibody (#APR-051), (1:60). A. Sections were incubated with Anti-PTH1R (extracellular) Antibody, followed by donkey-anti-rabbit-Cy3 (red). B. The same sections were incubated with Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody, (green). C. Merge of A and B shows staining of Orexin receptor 1 in nerve fibers (vertical arrows) while Parathyroid hormone receptor 1 is apparent on cell bodies (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Live cell imaging / Immunocytochemistry
  • Expression of Parathyroid hormone receptor 1 in rat U-87 MG cells
    Expression of Parathyroid hormone receptor 1 in rat U-87 MG cells
    Cell surface detection of Parathyroid hormone receptor 1 in intact living rat U-87 MG cells. A. Extracellular staining of cells using Anti-PTH1R (extracellular) Antibody (#APR-051), (1:50), (green). B. Merge of A with live view of the cells.
References
  1. Potts, J.T. and Gardella, T.J. (2007) Ann. N.Y. Acad. Sci. 1117, 196.
  2. Vilardaga, J.P. et al. (2011) Cell. Mol. Life Sci. 68, 1.
  3. Potts, J.T. (2005) J. Endocrinol. 187, 311.
  4. Chung, U.I. et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 13030.
  5. Vortkamp, A. et al. (1996) Science 273, 613.
  6. Mahon, M.J. et al. (2006) Mol. Endocrinol. 20, 136.
  7. Singh, A.T. et al. (2005) Endocrinology 146, 2171.
  8. Schipani, E. et al. (1995) Science 268, 98.
  9. Bastepe, M. et al. (2004) J. Clin. Endocrinol. Metab. 89, 3595.
  10. Duchatelet, S. et al. (2005) Hum. Mol. Genet. 14, 1.
Scientific background

The parathyroid hormone receptor type 1 (PTHR1) is bound and activated by two peptide hormones: the parathyroid hormone (PTH) secreted by the parathyroid gland and by parathyroid hormone related peptide (PTHrP) which is produced locally in many tissues. Although these two peptides bind the same receptor, they exert different actions. PTH regulates the concentrations of Ca2+, phosphate ions and vitamin D in the blood, stimulates bone formation/resorption and is distributed to the kidneys where it regulates Ca2+ and phosphate transport, and is also detected in the liver1-3. PTHrP, on the other hand, plays a critical role early in development of bone, heart, mammary glands and other tissues, and is detected in the circulation only during lactation and secreted by some tumors1,2,4,5. PTHR1 is obviously localized to all areas and tissues where PTH and PTHrP are known to exert their effects (i.e. bone, kidney, blood, heart, etc.).

PTHR1 belongs to the superfamily of G-protein coupled receptors. Like all members, PTHR1 has seven transmembrane spanning domains, an extracellular N-terminus and an intracellular C-terminal tail. Upon activation, the receptor couples to either Gs and Gq thereby activating adenylate cyclase and phospholipase C (PLC) respectively. In osteosarcoma cells, PTHR1 can also couple Gα2,6,7.

PTH is being used today to treat osteoporosis, as a factor promoting bone formation. Mutations in PTHR1 are associated with Jansen’s disease (hypercalcemia and dwarfism) and Eiken syndrome (cartilage tumors of bone)2,8-10.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Anti-PTH1R (extracellular) Antibody (#APR-051) is a highly specific antibody directed against an epitope of the rat Parathyroid hormone 1 receptor. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize PTH1R from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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