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Anti-Parkin Antibody

E3 Ubiquitin-Protein Ligase Parkin, Parkin RBR E3 ubiquitin-protein ligase, Parkinson Juvenile Disease Protein 2, PARK2, PRKN

Cat #: ANR-103
Alternative Name E3 Ubiquitin-Protein Ligase Parkin, Parkin RBR E3 ubiquitin-protein ligase, Parkinson Juvenile Disease Protein 2, PARK2, PRKN
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KELHHFRILGEEQYTR, corresponding to amino acid residues 298 - 313 of mouse Parkin (Accession Q9WVS6). Intracellular.
Accession (Uniprot) Number Q9WVS6
Gene ID 50873
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, Human - 15 out of 16 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain synaptosome preparation (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain synaptosome preparation (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1-2. Anti-Parkin Antibody (#ANR-103), (1:200).
    3-4. Anti-Parkin Antibody, preincubated with Parkin Blocking Peptide (BLP-NR103).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    1. Anti-Parkin Antibody (#ANR-103), (1:200).
    2. Anti-Parkin Antibody, preincubated with Parkin Blocking Peptide (BLP-NR103).
Immunohistochemistry
  • Expression of Parkin in rat  brain 4th ventricle.
    Expression of Parkin in rat  brain 4th ventricle.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Parkin Antibody (#ANR-103), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Parkin immunoreactivity (green) appears along the wall of the 4th cerebral ventricle (arrows). B. Pre-incubation of the antibody with Parkin Blocking Peptide (BLP-NR103), suppressed staining. Cell nuclei are stained with DAPI (blue). 4th V= 4th ventricle.
References
  1. Pohl, C. et al. (2019) Science., 366, 818.
  2. Hoeller, D. et al. (2016) Proc. Natl. Acad. Sci., 113, 13266.
  3. Panicker, N. et al. (2017) Boichem. J., 474, 3075.
  4. Murillo-González, F.E. et al. (2021) Biochem. Pharmacol., 190, 114650.
  5. George, J.A. et al. (2018) Front. Genet., 9,29.
Scientific background

Protein degradation is a major process in the cell, allowing control and adjustment of the levels of proteins. The enzyme responsible for the vast majority of cellular protein degradation is the 26S proteasome1. Protein degradation is a highly regulated process that dependents on specific poly-ubiquitination of the substrate. The ubiquitination pathway is catalyzed by E1, E2 and E3 enzymes that activate, conjugate, and ligate, respectively, the ubiquitin protein to the substrate protein2.

Parkin is a cytosolic E3 ubiquitin ligase that plays an important role in neuroprotection. Structurally, the Parkin protein is comprised of a ubiquitin-like (Ubl) domain at its N-terminus and a really interesting new gene (RING)1–in-between-ring (IBR) –RING2 domain and a zinc-chelating (RING0) domain at its C-terminus. E2 ubiquitin-conjugated enzymes are recruited to the RING1 domain where they transfer ubiquitin to the RING2 domain at the Parkin catalytic site. The ubiquitin is then transferred to the substrate protein, where it serves as a tag for recognition by the proteasome3.

Parkin activity prevent the accumulation and aggregation of damaged proteins and therefore it protects against unfolded protein stress. In addition, it is known to promote the elimination of aberrant mitochondria via mitophagy4. Mutations in Parkin have been associated with Parkinson’s disease (PD), Alzheimer’s disease and Huntington’s disease5

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 26/10/2021

Anti-Parkin Antibody (#ANR-103) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Parkin from rat, mouse and human samples.

For research purposes only, not for human use
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