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Anti-Platelet-activating factor receptor (extracellular) Antibody

PTAFR, PAFR

Cat #: APR-055
Alternative Name PTAFR, PAFR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)ELGYQTNFHQAINDAH, corresponding to amino acid residues 259 - 274 of mouse PTAFR (Accession Q62035). Extracellular, 3rd loop.
Accession (Uniprot) Number Q62035
Gene ID 19204
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry
Homology Rat – identical, Human – 13 out of 16 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1-2. Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:500).
    3-4. Anti-Platelet-activating factor receptor (extracellular) Antibody, preincubated with Platelet-activating factor receptor (extracellular) Blocking Peptide (BLP-PR055).
  • Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 2 and 4):
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 2 and 4):
    1-2. Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:500).
    3-4. Anti-Platelet-activating factor receptor (extracellular) Antibody, preincubated with Platelet-activating factor receptor (extracellular) Blocking Peptide (BLP-PR055).
Immunohistochemistry
  • Expression of Platelet-activating factor receptor in rat substantia nigra pars compacta (SNC).
    Expression of Platelet-activating factor receptor in rat substantia nigra pars compacta (SNC).
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. PAFR immunoreactivity (green) appears in SNC neurons (arrows). B. Pre-incubation of the antibody with Platelet-activating factor receptor (extracellular) Blocking Peptide (BLP-PR055), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of PAFR by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of PAFR by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055) (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Walterscheid, J.P. et al. (2002) J. Exp. Med., 195, 171.
  2. Cao, C. et al. (2018) Nat. Struct. Mol. Biol., 25, 488.
  3. Correa-Costa, M. et al. (2014) Lab. Invest., 94, 455.
  4. Melnikova, V. et al. (2007) Cancer. Metastasis. Rev., 26, 359.
Scientific background

Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a biologically active phospholipid mediator that was first described by its ability to cause platelet aggregation and dilation of blood vessels. In addition, PAF acts as a mediator of cell-to-cell communication, inflammation, allergic responses and shock1.

PAF acts on PAF receptor (PAFR) a G protein-coupled receptor (GPCR). It consists of 7 transmembrane-spanning domains (TMDs), and two potential N-linked glycosylation sites. PAFR was found to expressed in the lung, neutrophils, monocytes, macrophages, placenta, small intestine, heart, liver, kidney, brain, and spleen. As a results of PAF interaction, PAFR is coupled to Gq protein and consequently activates various downstream signaling pathways2.

Experiments in both PAF receptor knockout animals and transgenic animals overexpressing PAF receptors support the pathophysiological role attributed to PAF receptor signaling. For example, Mice model of obstructive nephropathy show that PAFR signaling contributes to a pro-inflammatory environment leading to renal dysfunction and progressive organ failure3. In addition, inhibition of PAFR signaling results in an effective inhibition of experimental tumor growth and metastasis4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 18/10/2021

Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize PAFR from rat, mouse and human samples.

For research purposes only, not for human use
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