Overview
- Peptide (C)ELGYQTNFHQAINDAH, corresponding to amino acid residues 259 - 274 of mouse PTAFR (Accession Q62035). Extracellular, 3rd loop.
Cell surface detection of PAFR by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Platelet-activating factor receptor (extracellular)-FITC Antibody (#APR-055-F), (2.5µg).
Cell surface detection of PAFR by direct flow cytometry in live intact mouse BV-2 microglia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Platelet-activating factor receptor (extracellular)-FITC Antibody (#APR-055-F), (2.5µg).
Cell surface detection of PAFR by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Platelet-activating factor receptor (extracellular)-FITC Antibody (#APR-055-F), (2.5µg).
Cell surface detection of PAFR by direct flow cytometry in live intact mouse P815 mastocytoma cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Platelet-activating factor receptor (extracellular)-FITC Antibody (#APR-055-F), (5µg).
- Walterscheid, J.P. et al. (2002) J. Exp. Med., 195, 171.
- Cao, C. et al. (2018) Nat. Struct. Mol. Biol., 25, 488.
- Correa-Costa, M. et al. (2014) Lab. Invest., 94, 455.
- Melnikova, V. et al. (2007) Cancer. Metastasis. Rev., 26, 359.
Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a biologically active phospholipid mediator that was first described by its ability to cause platelet aggregation and dilation of blood vessels. In addition, PAF acts as a mediator of cell-to-cell communication, inflammation, allergic responses and shock1.
PAF acts on PAF receptor (PAFR) a G protein-coupled receptor (GPCR). It consists of 7 transmembrane-spanning domains (TMDs), and two potential N-linked glycosylation sites. PAFR was found to expressed in the lung, neutrophils, monocytes, macrophages, placenta, small intestine, heart, liver, kidney, brain, and spleen. As a results of PAF interaction, PAFR is coupled to Gq protein and consequently activates various downstream signaling pathways2.
Experiments in both PAF receptor knockout animals and transgenic animals overexpressing PAF receptors support the pathophysiological role attributed to PAF receptor signaling. For example, Mice model of obstructive nephropathy show that PAFR signaling contributes to a pro-inflammatory environment leading to renal dysfunction and progressive organ failure3. In addition, inhibition of PAFR signaling results in an effective inhibition of experimental tumor growth and metastasis4.
Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize PAFR from rat, mouse and human samples.
Anti-Platelet-activating factor receptor (extracellular)-FITC Antibody (#APR-055-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize PAFR from rat, mouse and human samples.
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