Free shipping starts now, no minimum, no coupons required!

Anti-Plexin-A4 (extracellular) Antibody

PLXNA4

Cat #: APR-084
Alternative Name PLXNA4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RKLTKNSEADGMFAY, corresponding to amino acid residues 201 - 215 of mouse Plexin-A4 (Accession Q80UG2). Extracellular, N-term.
Accession (Uniprot) Number Q80UG2
Gene ID 243743
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, Rat – identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Plexin-A4 (extracellular) Blocking Peptide (#BLP-PR084)
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1-2. Anti-Plexin-A4 (extracellular) Antibody (#APR-084), (1:200).
    3-4. Anti-Plexin-A4 (extracellular) Antibody, preincubated with Plexin-A4 (extracellular) Blocking Peptide (BLP-PR084).
  • Western blot analysis of human THP-1 monocytic leukemia cell line lysate:
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate:
    1. Anti-Plexin-A4 (extracellular) Antibody (#APR-084), (1:200).
    2. Anti-Plexin-A4 (extracellular) Antibody, preincubated with Plexin-A4 (extracellular) Blocking Peptide (BLP-PR084).
Immunohistochemistry
  • Expression of Plexin-A4 in rat hippocampus.
    Expression of Plexin-A4 in rat hippocampus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Plexin-A4 (extracellular) Antibody (#APR-084), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal mossy fiber terminal zone (MFT), showed Plexin-A4 immunoreactivity (green) in a zone adjacent to the pyramidal layer (horizontal arrows). B. Pre-incubation of the antibody with Plexin-A4 (extracellular) Blocking Peptide (BLP-PR084), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of Plexin-A4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of Plexin-A4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Plexin-A4 (extracellular) Antibody (#APR-084), (5μg) + goat-anti-rabbit-FITC.
References
  1. Pascoe, H.G. et. al. (2015) Prog. Biophys. Mol. Biol., 118, 161.
  2. Lu, D. et. al. (2021) Nat. Commun., 12, 3172.
  3. Christie, M.S. et. al. (2021) J. Biol. Chem., 297, 100965.
  4. Han, Q. et. al. (2018) Front. Neurosci., 12, 946.
  5. Celus, W. et. al. (2022) Cancer Immunol. Res., 10, 126.
Scientific background

The plexin family are transmembrane proteins which are classified into four subfamilies: plexin -A, B, C and D. The plexins serve as a receptor for seamaphorins (Sema), axon guidance molecules involved in cellular processes such as axon pruning and repulsion, regulation of cell migration, vascular remodeling and more1

All family members consist of a Sema domain at the N-terminus responsible for Sema binding. In addition, plexins have PSI and IPT (Integrin, Plexin, and Transcription factor) domains, which connect to the transmembrane (TM) helix. Moreover, intracellular region of all plexins contains a membrane proximal juxtamembrane region (JM), a Rho GTPase-binding domain (RBD), and GTPase Activating Protein (GAP) domain2.

Plexin-A4 acts as a receptor for the secreted semaphorins Sema3A and Sema6A in the central nervous system, and mediates axon repulsion and synaptic plasticity. Moreover, genome-wide significant association study (GWAS) on humans, has identified that several single nucleotide polymorphisms (SNPs) in PLXNA4 can increase the risk of Alzheimer’s disease (AD) 3,4.

In the immune system, Plexin-A4 has been implicated in macrophage Toll-like receptor (TLR)–mediated signaling in sepsis, negative regulation of T-cell mediated immune responses in cancer5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 06/11/2022

Anti-Plexin-A4 (extracellular) Antibody (#APR-084) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Plexin-A4 from mouse, rat and human samples.

For research purposes only, not for human use
Shipping and Ordering information